Fast and cost‐efficient species identification of Atlantic salmon (Salmo salar L.), brown trout (Salmo trutta), and their hybrids using a single SNP marker
Abstract
A workflow for developing a cost- and time-efficient, single nucleotide polymorphism (SNP)-based assay for species and hybrid identification is described. In a reference set (n = 46), the developed assay identified individuals of two closely related species, the Atlantic salmon (Salmo salar L., n = 23) and brown trout (Salmo trutta, n = 23), with 100% accuracy. Furthermore, species and hybrid identification using field-collected embryos had 98.1% concordance (155/158) to more expensive and time-consuming methods that utilized multiple SNP markers. The method can be integrated into management and conservation plans to quantify species' spawning distribution and hybridization rates.
Main Authors
Format
Articles
Research article
Published
2024
Series
Subjects
Publication in research information system
Publisher
Wiley
The permanent address of the publication
https://urn.fi/URN:NBN:fi:jyu-202412187856Use this for linking
Review status
Peer reviewed
ISSN
0022-1112
DOI
https://doi.org/10.1111/jfb.16032
Language
English
Published in
Journal of Fish Biology
Citation
- Aykanat, T., Balatsou, A., Kähkönen, K., Syrjänen, J. T., Janhunen, M., Leinonen, T., Prokkola, J. M., Norrgård, J. R., & Piccolo, J. J. (2024). Fast and cost‐efficient species identification of Atlantic salmon (Salmo salar L.), brown trout (Salmo trutta), and their hybrids using a single SNP marker. Journal of Fish Biology, Early online. https://doi.org/10.1111/jfb.16032
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Additional information about funding
This study was funded by the Fortum Sverige AB (to J.T.S. and T.A.), the R. Erik and Bror Serlachius Foundation (to J.T.S.), the Centre for Economic Development, Transport and the Environment (fisheries funds of ELY Centre of North Savo; to M.J.), the Swedish Center for Sustainable Hydropower Project VKU31016 (to J.J.P.), and the Research Council of Finland (325964 to T.A., 348965 to J.M.P., 347368 to T.L.).
Copyright© 2024 The Author(s). Journal of Fish Biology published by John Wiley & Sons Ltd on behalf of Fisheries Society of the British Isles