Oldies, but goldies : preserved morphology and stability of antigenic determinants in decades-old cryosections of human m. vastus lateralis
Hutz, B., Degens, H., & Korhonen, M. T. (2024). Oldies, but goldies : preserved morphology and stability of antigenic determinants in decades-old cryosections of human m. vastus lateralis. Journal of Anatomy, Early View. https://doi.org/10.1111/joa.14003
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Journal of AnatomyDate
2024Copyright
© 2024 The Authors. Journal of Anatomy published by John Wiley & Sons Ltd on behalf of Anatomical Society
Fibre typing by immunohistochemistry on cryosections from human skeletal muscle biopsies is an essential tool in the diagnosis and research of muscular diseases, ageing, and responses to exercise training and disuse. Preserving a good quality in these frozen specimens can be challenging especially if they are stored for longer periods before histological processing, which is often the case in studies with a large number of test subjects and/or repeated sampling separated by multiple years. We demonstrate in this article that both, the morphology and reactivity of epitopes to myosin heavy chain isoforms and dystrophin are well preserved in up to 18-year-stored unfixed and unstained cryosections of human m. vastus lateralis (n = 241). Any variation in staining intensity between samples was unrelated to the age of the biopsy donor or the storage period of the unstained cryosections, and in all cases, the obtained images were appropriate for image analysis, such as the determination of the fibre type composition and the fibre cross-sectional area, and quantitative analysis of muscle capillarisation.
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Wiley-BlackwellISSN Search the Publication Forum
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- Liikuntatieteiden tiedekunta [3092]
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This study was supported in part by grants from the Juho Vainio Foundation, Finnish Ministry of Education and Culture, Yrjö Jahnsson Foundation, Päivikki and Sakari Sohlberg Foundation, and EU Socrates/Erasmus programme.License
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