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dc.contributor.authorChen, Jian-Hua
dc.contributor.authorVanslembrouck, Bieke
dc.contributor.authorEkman, Axel
dc.contributor.authorAho, Vesa
dc.contributor.authorLarabell, Carolyn A.
dc.contributor.authorLe Gros, Mark A.
dc.contributor.authorVihinen-Ranta, Maija
dc.contributor.authorWeinhardt, Venera
dc.date.accessioned2023-01-03T07:55:39Z
dc.date.available2023-01-03T07:55:39Z
dc.date.issued2022
dc.identifier.citationChen, J.-H., Vanslembrouck, B., Ekman, A., Aho, V., Larabell, C. A., Le Gros, M. A., Vihinen-Ranta, M., & Weinhardt, V. (2022). Soft X-ray Tomography Reveals HSV-1-Induced Remodeling of Human B Cells. <i>Viruses</i>, <i>14</i>(12), 2651. <a href="https://doi.org/10.3390/v14122651" target="_blank">https://doi.org/10.3390/v14122651</a>
dc.identifier.otherCONVID_164829491
dc.identifier.urihttps://jyx.jyu.fi/handle/123456789/84688
dc.description.abstractUpon infection, viruses hijack the cell machinery and remodel host cell structures to utilize them for viral proliferation. Since viruses are about a thousand times smaller than their host cells, imaging virus-host interactions at high spatial resolution is like looking for a needle in a haystack. Scouting gross cellular changes with fluorescent microscopy is only possible for well-established viruses, where fluorescent tagging is developed. Soft X-ray tomography (SXT) offers 3D imaging of entire cells without the need for chemical fixation or labeling. Here, we use full-rotation SXT to visualize entire human B cells infected by the herpes simplex virus 1 (HSV-1). We have mapped the temporospatial remodeling of cells during the infection and observed changes in cellular structures, such as the presence of cytoplasmic stress granules and multivesicular structures, formation of nuclear virus-induced dense bodies, and aggregates of capsids. Our results demonstrate the power of SXT imaging for scouting virus-induced changes in infected cells and understanding the orchestration of virus-host remodeling quantitatively.en
dc.format.mimetypeapplication/pdf
dc.language.isoeng
dc.publisherMDPI AG
dc.relation.ispartofseriesViruses
dc.rightsCC BY 4.0
dc.subject.otherX-ray tomography
dc.subject.othersoft X-rays
dc.subject.otherinfection imaging
dc.subject.otherHSV-1
dc.subject.othercell mapping
dc.subject.othercryo imaging
dc.titleSoft X-ray Tomography Reveals HSV-1-Induced Remodeling of Human B Cells
dc.typearticle
dc.identifier.urnURN:NBN:fi:jyu-202301031046
dc.contributor.laitosBio- ja ympäristötieteiden laitosfi
dc.contributor.laitosDepartment of Biological and Environmental Scienceen
dc.contributor.oppiaineSolu- ja molekyylibiologiafi
dc.contributor.oppiaineNanoscience Centerfi
dc.contributor.oppiaineCell and Molecular Biologyen
dc.contributor.oppiaineNanoscience Centeren
dc.type.urihttp://purl.org/eprint/type/JournalArticle
dc.type.coarhttp://purl.org/coar/resource_type/c_2df8fbb1
dc.description.reviewstatuspeerReviewed
dc.format.pagerange2651
dc.relation.issn1999-4915
dc.relation.numberinseries12
dc.relation.volume14
dc.type.versionpublishedVersion
dc.rights.copyright© 2022 by the authors. Licensee MDPI, Basel, Switzerland.
dc.rights.accesslevelopenAccessfi
dc.relation.grantnumber101017116
dc.relation.grantnumber101017116
dc.relation.grantnumber330896
dc.relation.grantnumber6420-1252f
dc.relation.grantnumber3741-e71e8
dc.relation.grantnumber
dc.relation.projectidinfo:eu-repo/grantAgreement/EC/H2020/101017116/EU//CoCID
dc.subject.ysovirukset
dc.subject.ysoisäntäsolut
dc.subject.ysoherpes simplex -virus
dc.subject.ysoinfektiot
dc.subject.ysotomografia
dc.subject.ysomikroskopia
dc.subject.ysosolubiologia
dc.subject.ysoherpesvirukset
dc.subject.ysosolut
dc.subject.ysoröntgenkuvaus
dc.subject.ysoherpesvirusinfektiot
dc.format.contentfulltext
jyx.subject.urihttp://www.yso.fi/onto/yso/p1123
jyx.subject.urihttp://www.yso.fi/onto/yso/p27923
jyx.subject.urihttp://www.yso.fi/onto/yso/p7738
jyx.subject.urihttp://www.yso.fi/onto/yso/p7316
jyx.subject.urihttp://www.yso.fi/onto/yso/p17798
jyx.subject.urihttp://www.yso.fi/onto/yso/p16290
jyx.subject.urihttp://www.yso.fi/onto/yso/p18492
jyx.subject.urihttp://www.yso.fi/onto/yso/p21634
jyx.subject.urihttp://www.yso.fi/onto/yso/p2409
jyx.subject.urihttp://www.yso.fi/onto/yso/p10181
jyx.subject.urihttp://www.yso.fi/onto/yso/p11103
dc.rights.urlhttps://creativecommons.org/licenses/by/4.0/
dc.relation.doi10.3390/v14122651
dc.relation.funderEuropean Commissionen
dc.relation.funderResearch Council of Finlanden
dc.relation.funderJane and Aatos Erkko Foundationen
dc.relation.funderJane and Aatos Erkko Foundationen
dc.relation.funderJane and Aatos Erkko Foundationen
dc.relation.funderEuroopan komissiofi
dc.relation.funderSuomen Akatemiafi
dc.relation.funderJane ja Aatos Erkon säätiöfi
dc.relation.funderJane ja Aatos Erkon säätiöfi
dc.relation.funderJane ja Aatos Erkon säätiöfi
jyx.fundingprogramRIA Research and Innovation Action, H2020en
jyx.fundingprogramAcademy Project, AoFen
jyx.fundingprogramFoundationen
jyx.fundingprogramFoundationen
jyx.fundingprogramFoundationen
jyx.fundingprogramRIA Research and Innovation Action, H2020fi
jyx.fundingprogramAkatemiahanke, SAfi
jyx.fundingprogramSäätiöfi
jyx.fundingprogramSäätiöfi
jyx.fundingprogramSäätiöfi
jyx.fundinginformationThe National Center for X-ray Tomography is supported by NIH NIGMS (P41GM103445, P30GM138441) and the DOE’s Office of Biological and Environmental Research (DE-AC025CH11231). V.W. was supported by the ERC Synergy Grant IndiGene (no. 810172 to Jochen Wittbrodt). M.V.-R. was supported by the Jane and Aatos Erkko Foundation and the Academy of Finland (grant number 330896). V.W. and M.V.-R. are supported by the CoCID project (no. 101017116) funded by EU Research and Innovation Act.
dc.type.okmA1


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