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dc.contributor.authorLautaoja, Juulia H.
dc.contributor.authorO'Connell, Thomas
dc.contributor.authorMäntyselkä, Sakari
dc.contributor.authorPeräkylä, Juuli
dc.contributor.authorKainulainen, Heikki
dc.contributor.authorPekkala, Satu
dc.contributor.authorPermi, Perttu
dc.contributor.authorHulmi, Juha J.
dc.date.accessioned2021-07-08T07:36:17Z
dc.date.available2021-07-08T07:36:17Z
dc.date.issued2021
dc.identifier.citationLautaoja, J. H., O'Connell, T., Mäntyselkä, S., Peräkylä, J., Kainulainen, H., Pekkala, S., Permi, P., & Hulmi, J. J. (2021). Higher glucose availability augments the metabolic responses of the C2C12 myotubes to exercise-like electrical pulse stimulation. <i>American Journal of Physiology : Endocrinology and Metabolism</i>, <i>321</i>(2), E229-E245. <a href="https://doi.org/10.1152/ajpendo.00133.2021" target="_blank">https://doi.org/10.1152/ajpendo.00133.2021</a>
dc.identifier.otherCONVID_98406059
dc.identifier.urihttps://jyx.jyu.fi/handle/123456789/77055
dc.description.abstractThe application of exercise-like electrical pulse simulation (EL-EPS) has become a widely used exercise mimetic in vitro. EL-EPS produces similar physiological responses as in vivo exercise, while less is known about the detailed metabolic effects. Routinely the C2C12 myotubes are cultured in high glucose medium (4.5 g/l), which may alter EL-EPS responses. In this study, we evaluate the metabolic effects of EL-EPS under the high and low glucose (1.0 g/l) conditions to understand how substrate availability affects the myotube response to EL-EPS.The C2C12 myotube, media and cell-free media metabolites were analyzed using untargeted nuclear magnetic resonance (NMR)-based metabolomics. Further, translational and metabolic changes and possible exerkine effects were analyzed. EL-EPS enhanced substrate utilization as well as production and secretion of lactate, acetate, 3-hydroxybutyrate and branched chain fatty acids (BCFAs). The increase in BCFAs correlated with branched chain amino acids (BCAAs) and BCFAs were strongly decreased when myotubes were cultured without BCAAs suggesting the action of acyl-CoA thioesterases on BCAA catabolites. Notably, not all EL-EPS responses were augmented by high glucose because EL-EPS increased phosphorylated c-Jun N-terminal kinase and interleukin-6 secretion independent of glucose availability. Administration of acetate and EL-EPS conditioned media on HepG2 hepatocytes had no adverse effects on lipolysis or triacylglycerol content.Our results demonstrate that unlike in cell-free media, the C2C12 myotube and media metabolites were affected by EL-EPS, particularly under high glucose condition suggesting that media composition should be considered in future EL-EPS studies. Further, acetate and BCFAs were identified as putative exerkines warranting more research.en
dc.format.mimetypeapplication/pdf
dc.language.isoeng
dc.publisherAmerican Physiological Society
dc.relation.ispartofseriesAmerican Journal of Physiology : Endocrinology and Metabolism
dc.rightsIn Copyright
dc.subject.otheracetate
dc.subject.otherexerkine
dc.subject.othermetabolomics
dc.subject.otherskeletal muscle
dc.subject.otherbranched chain fatty acids
dc.titleHigher glucose availability augments the metabolic responses of the C2C12 myotubes to exercise-like electrical pulse stimulation
dc.typearticle
dc.identifier.urnURN:NBN:fi:jyu-202107084241
dc.contributor.laitosBio- ja ympäristötieteiden laitosfi
dc.contributor.laitosLiikuntatieteellinen tiedekuntafi
dc.contributor.laitosDepartment of Biological and Environmental Scienceen
dc.contributor.laitosFaculty of Sport and Health Sciencesen
dc.contributor.oppiaineLiikuntalääketiedefi
dc.contributor.oppiaineLiikuntafysiologiafi
dc.contributor.oppiaineNanoscience Centerfi
dc.contributor.oppiaineSports and Exercise Medicineen
dc.contributor.oppiaineExercise Physiologyen
dc.contributor.oppiaineNanoscience Centeren
dc.type.urihttp://purl.org/eprint/type/JournalArticle
dc.type.coarhttp://purl.org/coar/resource_type/c_2df8fbb1
dc.description.reviewstatuspeerReviewed
dc.format.pagerangeE229-E245
dc.relation.issn0193-1849
dc.relation.numberinseries2
dc.relation.volume321
dc.type.versionacceptedVersion
dc.rights.copyright© 2021, American Journal of Physiology-Endocrinology and Metabolism
dc.rights.accesslevelopenAccessfi
dc.relation.grantnumber308042
dc.relation.grantnumber275922
dc.relation.grantnumber323435
dc.relation.grantnumber298875
dc.subject.ysorasvahapot
dc.subject.ysoglukoosi
dc.subject.ysoaineenvaihdunta
dc.subject.ysolihassolut
dc.subject.ysoaineenvaihduntatuotteet
dc.subject.ysoasetaatit
dc.subject.ysolaktaatit
dc.subject.ysosolufysiologia
dc.subject.ysoin vitro -menetelmä
dc.format.contentfulltext
jyx.subject.urihttp://www.yso.fi/onto/yso/p4800
jyx.subject.urihttp://www.yso.fi/onto/yso/p18742
jyx.subject.urihttp://www.yso.fi/onto/yso/p3066
jyx.subject.urihttp://www.yso.fi/onto/yso/p25540
jyx.subject.urihttp://www.yso.fi/onto/yso/p24583
jyx.subject.urihttp://www.yso.fi/onto/yso/p8312
jyx.subject.urihttp://www.yso.fi/onto/yso/p9014
jyx.subject.urihttp://www.yso.fi/onto/yso/p25367
jyx.subject.urihttp://www.yso.fi/onto/yso/p21041
dc.rights.urlhttp://rightsstatements.org/page/InC/1.0/?language=en
dc.rights.accessrights
dc.relation.doi10.1152/ajpendo.00133.2021
dc.relation.funderResearch Council of Finlanden
dc.relation.funderResearch Council of Finlanden
dc.relation.funderResearch Council of Finlanden
dc.relation.funderResearch Council of Finlanden
dc.relation.funderSuomen Akatemiafi
dc.relation.funderSuomen Akatemiafi
dc.relation.funderSuomen Akatemiafi
dc.relation.funderSuomen Akatemiafi
jyx.fundingprogramAcademy Research Fellow, AoFen
jyx.fundingprogramAcademy Research Fellow, AoFen
jyx.fundingprogramAcademy Project, AoFen
jyx.fundingprogramAcademy Project, AoFen
jyx.fundingprogramAkatemiatutkija, SAfi
jyx.fundingprogramAkatemiatutkija, SAfi
jyx.fundingprogramAkatemiahanke, SAfi
jyx.fundingprogramAkatemiahanke, SAfi
jyx.fundinginformationThis work was funded by the Academy of Finland (Grant No. 298875 to H.K, 308042 to S.P., 323435 to P.P and 275922 to J.J.H.). T.M.O. is supported by grants from National Institutes of Health, National Institute of Arthritis and Musculoskeletal and Skin Diseases (P01AG039355 and P30AR072581) as well as the Additional Ventures, Single Ventricle Research Fund.
dc.type.okmA1


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