Imaging Bacterial Colonies and Phage-Bacterium Interaction at Sub-Nanometer Resolution Using Helium-Ion Microscopy
Leppänen, M., Sundberg, L.-R., Laanto, E., De Freitas Almeida, G., Papponen, P., & Maasilta, I. (2017). Imaging Bacterial Colonies and Phage-Bacterium Interaction at Sub-Nanometer Resolution Using Helium-Ion Microscopy. Advanced Biosystems, 1(8), Article 1700070. https://doi.org/10.1002/adbi.201700070
Published inAdvanced Biosystems
DisciplineSolu- ja molekyylibiologiaFysiikkaBiologisten vuorovaikutusten huippututkimusyksikköNanoscience CenterCell and Molecular BiologyPhysicsCentre of Excellence in Biological Interactions ResearchNanoscience Center
© 2017 WILEY‐VCH Verlag GmbH & Co
Imaging of microbial interactions has so far been based on well‐established electron microscopy methods. This study presents a new way to study bacterial colonies and interactions between bacteria and their viruses, bacteriophages (phages), in situ on agar plates using helium ion microscopy (HIM). In biological imaging, HIM has advantages over traditional scanning electron microscopy with its sub‐nanometer resolution, increased surface sensitivity, and the possibility to image nonconductive samples. Furthermore, by controlling the He beam dose or by using heavier Ne ions, the HIM instrument provides the possibility to mill out material in the samples, allowing for subsurface imaging and in situ sectioning. Here, the first HIM‐images of bacterial colonies and phage–bacterium interactions are presented at different stages of the infection as they occur on an agar culture. The feasibility of neon and helium milling is also demonstrated to reveal the subsurface structures of bacterial colonies on agar substrate, and in some cases also structure inside individual bacteria after cross‐sectioning. The study concludes that HIM offers great opportunities to advance the studies of microbial imaging, in particular in the area of interaction of viruses with cells. ...
Publication in research information system
MetadataShow full item record
Related funder(s)Academy of Finland
Funding program(s)Research post as Academy Research Fellow, AoF
Additional information about fundingFinnish Centre of Excellence Program of the Academy of Finland CoE in Biological Interactions. Grant Number: 2012‐2017 (#252411) Academy of Finland. Grant Number: #266879
Showing items with similar title or keywords.
Resolving Bio-Nano Interactions of E.coli Bacteria-Dragonfly Wing Interface with Helium Ion and 3D-Structured Illumination Microscopy to Understand Bacterial Death on Nanotopography Bandara, Chaturanga D.; Ballerin, Giulia; Leppänen, Miika; Tesfamichael, Tuquabo; Ostrikov, Kostya (Ken); Whitchurch, Cynthia B. (American Chemical Society, 2020)Obtaining a comprehensive understanding of the bactericidal mechanisms of natural nanotextured surfaces is crucial for the development of fabricated nanotextured surfaces with efficient bactericidal activity. However, the ...
Research data of an article: "Application of high resolution melting assay (HMR) to study temperature dependent infraspecific competition in an pathogenic bacterium." Ashrafi, Roghaieh; Bruneaux, Matthieu; Sundberg, Lotta-Riina; Pulkkinen, Katja; Ketola, Tarmo (Scientific Reports, 2017)Studies on species’ responses to climate change have focused largely on the direct effect of abiotic factors and in particular temperature, neglecting the effects of biotic interactions in determining the outcome of climate ...
Application of high resolution melting assay (HRM) to study temperature-dependent intraspecific competition in a pathogenic bacterium Ashrafi, Roghaieh; Bruneaux, Matthieu; Sundberg, Lotta-Riina; Pulkkinen, Katja; Ketola, Tarmo (Nature Publishing Group, 2017)Studies on species’ responses to climate change have focused largely on the direct effect of abiotic factors and in particular temperature, neglecting the effects of biotic interactions in determining the outcome of climate ...
Schmidt, Matthias; Byrne, James M.; Maasilta, Ilari J. (Beilstein-Institut zur Förderung der Chemischen Wissenschaften, 2021)Scanning helium-ion microscopy (HIM) is an imaging technique with sub-nanometre resolution and is a powerful tool to resolve some of the tiniest structures in biology. In many aspects, the HIM resembles a field-emission ...
Bacterial and phytoplankton responses to nutrient amendments in a boreal lake differ according to season and to taxonomic resolution Peura, Sari; Eiler, Alexander; Hiltunen, Minna; Nykänen, Hannu; Tiirola, Marja; Jones, Roger (Public Library of Science, 2012)Nutrient limitation and resource competition in bacterial and phytoplankton communities may appear different when considering different levels of taxonomic resolution. Nutrient amendment experiments conducted in a boreal ...