Näytä suppeat kuvailutiedot

dc.contributor.authorPeltomaa, Elina T.
dc.contributor.authorTaipale, Sami
dc.date.accessioned2020-01-14T10:28:23Z
dc.date.available2020-01-14T10:28:23Z
dc.date.issued2020
dc.identifier.citationPeltomaa, E. T., & Taipale, S. (2020). Osmotrophic glucose and leucine assimilation and its impact on EPA and DHA content in algae. <i>PeerJ</i>, <i>8</i>, Article e8363. <a href="https://doi.org/10.7717/peerj.8363" target="_blank">https://doi.org/10.7717/peerj.8363</a>
dc.identifier.otherCONVID_34045170
dc.identifier.urihttps://jyx.jyu.fi/handle/123456789/67267
dc.description.abstractThe uptake of dissolved organic compounds, that is, osmotrophy, has been shown to be an efficient nutritional strategy for algae. However, this mode of nutrition may affect the biochemical composition, for example, the fatty acid (FA) contents, of algal cells. This study focused on the osmotrophic assimilation of glucose and leucine by selected seven algal strains belonging to chlorophytes, chrysophytes, cryptophytes, dinoflagellates and euglenoids. Our laboratory experiments with stable isotope labeling showed that osmotrophy occurred in four of the selected seven strains. However, only three of these produced long chain omega-3 FAs eicosapentaenoic acid (EPA; 20:5ω3) and docosahexaenoic acid (DHA; 22:6ω3). High glucose content (5 mg L−1) affected negatively on the total FAs of Mallomonas kalinae and the total omega-3 FAs of Cryptomonas sp. Further, glucose assimilation explained 35% (negative effect) and leucine assimilation 48% (positive effect) of the variation of EPA, DHA and the FAs related to their synthesis in Cryptomonas sp. Moderate glucose concentration (2 mg L−1) was found to enhance the growth of Cryptomonas ozolinii, whereas low leucine (20 µg L−1) enhanced the growth of M. kalinae. However, no systematic effect of osmotrophy on growth rates was detected. Our study shows that osmotrophic assimilation of algae is species and compound specific, and that the effects of the assimilated compounds on algal metabolism also varies depending on the species.en
dc.format.mimetypeapplication/pdf
dc.languageeng
dc.language.isoeng
dc.publisherPeerJ, Ltd.
dc.relation.ispartofseriesPeerJ
dc.rightsCC BY 4.0
dc.subject.othermixotrophy
dc.subject.otheromega-3 fatty acids
dc.subject.otherstable isotope labeling
dc.subject.othercryptophytes
dc.titleOsmotrophic glucose and leucine assimilation and its impact on EPA and DHA content in algae
dc.typearticle
dc.identifier.urnURN:NBN:fi:jyu-202001141220
dc.contributor.laitosBio- ja ympäristötieteiden laitosfi
dc.contributor.laitosDepartment of Biological and Environmental Scienceen
dc.contributor.oppiaineAkvaattiset tieteetfi
dc.contributor.oppiaineAquatic Sciencesen
dc.type.urihttp://purl.org/eprint/type/JournalArticle
dc.type.coarhttp://purl.org/coar/resource_type/c_2df8fbb1
dc.description.reviewstatuspeerReviewed
dc.relation.issn2167-8359
dc.relation.volume8
dc.type.versionpublishedVersion
dc.rights.copyright© 2020, Peltomaa and Taipale
dc.rights.accesslevelopenAccessfi
dc.subject.ysolevät
dc.subject.ysoomegarasvahapot
dc.subject.ysoisotoopit
dc.format.contentfulltext
jyx.subject.urihttp://www.yso.fi/onto/yso/p3327
jyx.subject.urihttp://www.yso.fi/onto/yso/p23183
jyx.subject.urihttp://www.yso.fi/onto/yso/p6387
dc.rights.urlhttps://creativecommons.org/licenses/by/4.0/
dc.relation.datasethttp://dx.doi.org/10.7717/ peerj.8363#supplemental-information
dc.relation.doi10.7717/peerj.8363
jyx.fundinginformationThis work was supported by the Academy of Finland (No. 276268).
dc.type.okmA1


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