dc.contributor.author | Lemopoulos, Alexandre | |
dc.contributor.author | Prokkola, Jenni M. | |
dc.contributor.author | Uusi-Heikkilä, Silva | |
dc.contributor.author | Vasemägi, Anti | |
dc.contributor.author | Huusko, Ari | |
dc.contributor.author | Hyvärinen, Pekka | |
dc.contributor.author | Koljonen, Marja-Liisa | |
dc.contributor.author | Koskiniemi, Jarmo | |
dc.contributor.author | Vainikka, Anssi | |
dc.date.accessioned | 2019-03-18T11:14:27Z | |
dc.date.available | 2019-03-18T11:14:27Z | |
dc.date.issued | 2019 | |
dc.identifier.citation | Lemopoulos, A., Prokkola, J. M., Uusi-Heikkilä, S., Vasemägi, A., Huusko, A., Hyvärinen, P., Koljonen, M.-L., Koskiniemi, J., & Vainikka, A. (2019). Comparing RADseq and microsatellites for estimating genetic diversity and relatedness : Implications for brown trout conservation. <i>Nature Ecology and Evolution</i>, <i>9</i>(4), 2106-2120. <a href="https://doi.org/10.1002/ece3.4905" target="_blank">https://doi.org/10.1002/ece3.4905</a> | |
dc.identifier.other | CONVID_28906546 | |
dc.identifier.other | TUTKAID_80573 | |
dc.identifier.uri | https://jyx.jyu.fi/handle/123456789/63193 | |
dc.description.abstract | The conservation and management of endangered species requires information on
their genetic diversity, relatedness and population structure. The main genetic markers applied for these questions are microsatellites and single nucleotide polymorphisms (SNPs), the latter of which remain the more resource demanding approach in
most cases. Here, we compare the performance of two approaches, SNPs obtained
by restriction‐site‐associated DNA sequencing (RADseq) and 16 DNA microsatellite
loci, for estimating genetic diversity, relatedness and genetic differentiation of three,
small, geographically close wild brown trout (Salmo trutta) populations and a regionally used hatchery strain. The genetic differentiation, quantified as FST, was similar
when measured using 16 microsatellites and 4,876 SNPs. Based on both marker
types, each brown trout population represented a distinct gene pool with a low level
of interbreeding. Analysis of SNPs identified half‐ and full‐siblings with a higher probability than the analysis based on microsatellites, and SNPs outperformed microsatellites in estimating individual‐level multilocus heterozygosity. Overall, the results
indicated that moderately polymorphic microsatellites and SNPs from RADseq
agreed on estimates of population genetic structure in moderately diverged, small
populations, but RADseq outperformed microsatellites for applications that required
individual‐level genotype information, such as quantifying relatedness and individual‐level heterozygosity. The results can be applied to other small populations with
low or moderate levels of genetic diversity. | fi |
dc.format.mimetype | application/pdf | |
dc.language.iso | eng | |
dc.publisher | John Wiley & Sons Ltd. | |
dc.relation.ispartofseries | Nature Ecology and Evolution | |
dc.rights | CC BY 4.0 | |
dc.subject.other | ddRADseq | |
dc.subject.other | fisheries | |
dc.subject.other | relatedness | |
dc.subject.other | salmonids | |
dc.title | Comparing RADseq and microsatellites for estimating genetic diversity and relatedness : Implications for brown trout conservation | |
dc.type | article | |
dc.identifier.urn | URN:NBN:fi:jyu-201903131857 | |
dc.contributor.laitos | Bio- ja ympäristötieteiden laitos | fi |
dc.contributor.laitos | Department of Biological and Environmental Science | en |
dc.contributor.oppiaine | Akvaattiset tieteet | fi |
dc.contributor.oppiaine | Aquatic Sciences | en |
dc.type.uri | http://purl.org/eprint/type/JournalArticle | |
dc.date.updated | 2019-03-13T16:15:11Z | |
dc.type.coar | http://purl.org/coar/resource_type/c_2df8fbb1 | |
dc.description.reviewstatus | peerReviewed | |
dc.format.pagerange | 2106-2120 | |
dc.relation.issn | 2045-7758 | |
dc.relation.numberinseries | 4 | |
dc.relation.volume | 9 | |
dc.type.version | publishedVersion | |
dc.rights.copyright | © 2019 The Authors. | |
dc.rights.accesslevel | openAccess | fi |
dc.subject.yso | populaatiogenetiikka | |
dc.subject.yso | geneettinen monimuotoisuus | |
dc.subject.yso | DNA-analyysi | |
dc.subject.yso | kalanviljely | |
dc.subject.yso | taimen | |
dc.format.content | fulltext | |
jyx.subject.uri | http://www.yso.fi/onto/yso/p9005 | |
jyx.subject.uri | http://www.yso.fi/onto/yso/p27238 | |
jyx.subject.uri | http://www.yso.fi/onto/yso/p25695 | |
jyx.subject.uri | http://www.yso.fi/onto/yso/p5096 | |
jyx.subject.uri | http://www.yso.fi/onto/yso/p13633 | |
dc.rights.url | https://creativecommons.org/licenses/by/4.0/ | |
dc.relation.doi | 10.1002/ece3.4905 | |
dc.type.okm | A1 | |