Calpains promote α2β1 integrin turnover in non-recycling integrin pathway.
Rintanen, N., Karjalainen, M., Alanko, J., Paavolainen, L., Mäki, A., Nissinen, L., Lehkonen, M., Kallio, K., Cheng, R. H., Upla, P., Ivaska, J., & Marjomäki, V. (2012). Calpains promote α2β1 integrin turnover in non-recycling integrin pathway.. Molecular Biology of the Cell, 23(3), 448-463. https://doi.org/10.1091/mbc.e11-06-0548
Published in
Molecular Biology of the CellAuthors
Mäki, A. |
Date
2012Copyright
© 2012 Rintanen et al.
Collagen receptor integrins recycle between the plasma membrane and endosomes and facilitate formation and turnover of focal adhesions. In contrast, clustering of α2β1 integrin with antibodies or the human pathogen echovirus 1 (EV1) causes redistribution of α2 integrin to perinuclear multivesicular bodies, α2-MVBs. We show here that the internalized clustered α2 integrin remains in α2-MVBs and is not recycled back to the plasma membrane. Instead, receptor clustering and internalization lead to an accelerated down-regulation of α2β1 integrin compared to the slow turnover of unclustered α2 integrin. EV1 infection or integrin degradation is not associated with proteasomal or autophagosomal processes and shows no significant association with lysosomal pathway. In contrast, degradation is dependent on calpains, such that it is blocked by calpain inhibitors. We show that active calpain is present in α2-MVBs, internalized clustered α2β1 integrin coprecipitates with calpain-1, and calpain enzymes can degrade α2β1 integrin. In conclusion, we identified a novel virus- and clustering-specific pathway that diverts α2β1 integrin from its normal endo/exocytic traffic to a nonrecycling, calpain-dependent degradative endosomal route.
...
Publisher
American Society for Cell BiologyISSN Search the Publication Forum
1059-1524Publication in research information system
https://converis.jyu.fi/converis/portal/detail/Publication/20911413
Metadata
Show full item recordCollections
License
Related items
Showing items with similar title or keywords.
-
Cholesterol Dependence of Collagen and Echovirus 1 Trafficking along the Novel alpha2beta1 Integrin Internalization Pathway
Siljamäki, Elina; Rintanen, Nina; Kirsi, Maija; Upla, Paula; Wang, Wei; Karjalainen, Mikko; Ikonen, Elina; Marjomäki, Varpu (Public Library of Science, 2013)We have previously shown that soluble collagen and a human pathogen, echovirus 1 (EV1) cluster α2β1 integrin on the plasma membrane and cause their internalization into cytoplasmic endosomes. Here we show that cholesterol ... -
Clustering-triggered endocytic pathway of α2β1 integrin
Rintanen, Nina (University of Jyväskylä, 2012) -
The role of calpains in enterovirus infection
Kangasniemi, Niko (2019)Enterovirukset ovat viruspeitteettömien, positiivisten RNA-virusten ryhmään kuuluva virusperhe, jonka jäsenet aiheuttamat mitä monituisimpia tauteja suu- ja sorkkataudista aina aivokalvontulehdukseen. Aikaisemmat ... -
Echovirus 1 infectious entry via novel multivesicular bodies
Karjalainen, Mikko (University of Jyväskylä, 2011) -
Endosomin sisäisen ioniympäristön vaikutukset echovirus 1 -infektioon
Kallio, Katri (2009)