Evaluating polymicrobial immune responses in patients suffering from tick-borne diseases
Garg, K., Meriläinen, L., Franz, O., Pirttinen, H., Quevedo-Diaz, M., Croucher, S., & Gilbert, L. (2018). Evaluating polymicrobial immune responses in patients suffering from tick-borne diseases. Scientific Reports, 8, 15932. doi:10.1038/s41598-018-34393-9
Published inScientific Reports
DisciplineSolu- ja molekyylibiologia
© Te Authors 2018
There is insufcient evidence to support screening of various tick-borne diseases (TBD) related microbes alongside Borrelia in patients sufering from TBD. To evaluate the involvement of multiple microbial immune responses in patients experiencing TBD we utilized enzyme-linked immunosorbent assay. Four hundred and thirty-two human serum samples organized into seven categories followed Centers for Disease Control and Prevention two-tier Lyme disease (LD) diagnosis guidelines and Infectious Disease Society of America guidelines for post-treatment Lyme disease syndrome. All patient categories were tested for their immunoglobulin M (IgM) and G (IgG) responses against 20 microbes associated with TBD. Our fndings recognize that microbial infections in patients sufering from TBDs do not follow the one microbe, one disease Germ Theory as 65% of the TBD patients produce immune responses to various microbes. We have established a causal association between TBD patients and TBD associated co-infections and essential opportunistic microbes following Bradford Hill’s criteria. This study indicated an 85% probability that a randomly selected TBD patient will respond to Borrelia and other related TBD microbes rather than to Borrelia alone. A paradigm shift is required in current healthcare policies to diagnose TBD so that patients can get tested and treated even for opportunistic infections. ...