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dc.contributor.authorMäki, Anita
dc.contributor.authorRissanen, Antti
dc.contributor.authorTiirola, Marja
dc.date.accessioned2018-11-02T06:27:27Z
dc.date.available2018-11-02T06:27:27Z
dc.date.issued2016
dc.identifier.citationMäki, A., Rissanen, A., & Tiirola, M. (2016). A practical method for barcoding and size-trimming PCR templates for amplicon sequencing. <i>BioTechniques</i>, <i>60</i>(2), 88-90. <a href="https://doi.org/10.2144/000114380" target="_blank">https://doi.org/10.2144/000114380</a>
dc.identifier.otherCONVID_25548717
dc.identifier.otherTUTKAID_69188
dc.identifier.urihttps://jyx.jyu.fi/handle/123456789/60082
dc.description.abstractSample barcoding facilitates the analysis of tens or even hundreds of samples in a single next-generation sequencing (NGS) run, but more efficient methods are needed for high-throughput barcoding and size-trimming of long PCR products. Here we present a two-step PCR approach for barcoding followed by pool shearing, adapter ligation, and 5' end selection for trimming sets of DNA templates of any size. Our new trimming method offers clear benefits for phylogenetic studies, since targeting exactly the same region maximizes the alignment and enables the use of operational taxonomic unit (OTU)-based algorithms.fi
dc.format.mimetypeapplication/pdf
dc.language.isoeng
dc.publisherInforma Healthcare
dc.relation.ispartofseriesBioTechniques
dc.rightsCC BY 4.0
dc.subject.otherligation
dc.subject.othernext-generation sequencing
dc.subject.otherPCR
dc.subject.otherpolyclonality
dc.subject.otherprimer
dc.titleA practical method for barcoding and size-trimming PCR templates for amplicon sequencing
dc.typearticle
dc.identifier.urnURN:NBN:fi:jyu-201811014600
dc.contributor.laitosBio- ja ympäristötieteiden laitosfi
dc.contributor.laitosDepartment of Biological and Environmental Scienceen
dc.contributor.oppiaineAkvaattiset tieteetfi
dc.contributor.oppiaineAquatic Sciencesen
dc.type.urihttp://purl.org/eprint/type/JournalArticle
dc.date.updated2018-11-01T13:15:13Z
dc.type.coarhttp://purl.org/coar/resource_type/c_2df8fbb1
dc.description.reviewstatuspeerReviewed
dc.format.pagerange88-90
dc.relation.issn0736-6205
dc.relation.numberinseries2
dc.relation.volume60
dc.type.versionpublishedVersion
dc.rights.copyright© The Author(s) 2016.
dc.rights.accesslevelopenAccessfi
dc.format.contentfulltext
dc.rights.urlhttps://creativecommons.org/licenses/by/4.0/
dc.relation.doi10.2144/000114380
dc.type.okmA1


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Except where otherwise noted, this item's license is described as CC BY 4.0