Design and protocol of Estrogenic Regulation of Muscle Apoptosis (ERMA) study with 47 to 55-year-old women's cohort : novel results show menopause-related differences in blood count
Kovanen, V., Aukee, P., Kokko, K., Finni Juutinen, T., Tarkka, I., Tammelin, T., Kujala, U., Sipilä, S., & Laakkonen, E. (2018). Design and protocol of Estrogenic Regulation of Muscle Apoptosis (ERMA) study with 47 to 55-year-old women's cohort : novel results show menopause-related differences in blood count. Menopause: The Journal of The North American Menopause Society, 25(9), 1020-1032. https://doi.org/10.1097/GME.0000000000001117
DisciplineBiomekaniikkaGerontologia ja kansanterveysLiikuntalääketiedeBiomechanicsGerontology and Public HealthSports and Exercise Medicine
© 2018 the Authors
Objective: The multidisciplinary Estrogenic Regulation of Muscle Apoptosis (ERMA) study was designed to reveal how hormonal differences over the menopausal stages affect the physiological and psychological functioning of middle-aged women. This paper describes the protocol and nonrespondent analysis of ERMA and novel findings on menopausal differences in blood count variables and their association with female sex hormones. Methods: Women aged 47 to 55 years were assigned to pre, early peri, late peri, and postmenopausal groups based on follicle-stimulating hormone (FSH) and bleeding diary. Multivariate linear regression models were constructed to estimate the association of 17b-estradiol (E2) and FSH with the blood count variables. Results: In all, 3,064 women returned the prequestionnaire (ERMA phase one), 1,393 donated blood samples and were assigned to the relevant menopausal group (phase two), and 914 completed phase three, which included physiological and psychological measurements. Nonrespondents were more likely than respondents to be obese, whereas the menopausal groups showed no mean differences in body mass index. Blood count variables, while being within clinical reference values, showed significant differences between groups. E2 and FSH were associated with the white blood cell (WBC) count and neutrophil-to-lymphocyte ratio. Conclusions: The ERMA study was successful in recruiting and characterizing the menopausal status of a cohort sample of middle-aged women. The significant group differences found in the blood count variables and their associations with E2 and FSH verifies menopause-associated changes in WBC composition potentially being an early sign of low-grade inflammation that may develop later in life. Key Words: 17b-Estradiol – Blood viscosity – FSH – Leucocyte count – Menopausal status – Neutrophil- to-lymphocyte ratio. ...
PublisherLippincott Williams & Wilkins
Publication in research information system
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- Liikuntatieteiden tiedekunta 
Related funder(s)European Commission; Academy of Finland
Funding program(s)Academy Project, AoF; Research post as Academy Research Fellow, AoF
The content of the publication reflects only the author’s view. The funder is not responsible for any use that may be made of the information it contains.
Additional information about fundingThis research is supported by the Academy of Finland (VK: Grant nro 275323, EKL: nro 309504), PANINI (SS: Horizon 2020-the Framework Programme for Research and Innovation, Marie Sklodowska-Curie Actions, ITN, ref 15-0667), and the Juho Vainio Foundation (EKL). Financial disclosure/conflicts of interest: None reported.
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