Show simple item record

dc.contributor.authorPeura, Sari
dc.contributor.authorBuck, Moritz
dc.contributor.authorAalto, Sanni L.
dc.contributor.authorMorales, Sergio E.
dc.contributor.authorNykänen, Hannu
dc.contributor.authorEiler, Alexander
dc.date.accessioned2018-08-20T10:23:02Z
dc.date.available2018-08-20T10:23:02Z
dc.date.issued2018
dc.identifier.citationPeura, S., Buck, M., Aalto, S. L., Morales, S. E., Nykänen, H., & Eiler, A. (2018). Novel Autotrophic Organisms Contribute Significantly to the Internal Carbon Cycling Potential of a Boreal Lake. <i>mBio</i>, <i>9</i>(4), Article e00916-18. <a href="https://doi.org/10.1128/mBio.00916-18" target="_blank">https://doi.org/10.1128/mBio.00916-18</a>
dc.identifier.otherCONVID_28213553
dc.identifier.otherTUTKAID_78539
dc.identifier.urihttps://jyx.jyu.fi/handle/123456789/59277
dc.description.abstractOxygen-stratified lakes are typical for the boreal zone and also a major source of greenhouse gas emissions in the region. Due to shallow light penetration, restricting the growth of phototrophic organisms, and large allochthonous organic carbon inputs from the catchment area, the lake metabolism is expected to be dominated by heterotrophic organisms. In this study, we test this assumption and show that the potential for autotrophic carbon fixation and internal carbon cycling is high throughout the water column. Further, we show that during the summer stratification carbon fixation can exceed respiration in a boreal lake even below the euphotic zone. Metagenome-assembled genomes and 16S profiling of a vertical transect of the lake revealed multiple organisms in an oxygen-depleted compartment belonging to novel or poorly characterized phyla. Many of these organisms were chemolithotrophic, potentially deriving their energy from reactions related to sulfur, iron, and nitrogen transformations. The community, as well as the functions, was stratified along the redox gradient. The autotrophic potential in the lake metagenome below the oxygenic zone was high, pointing toward a need for revising our concepts of internal carbon cycling in boreal lakes. Further, the importance of chemolithoautotrophy for the internal carbon cycling suggests that many predicted climate changeassociated fluctuations in the physical properties of the lake, such as altered mixing patterns, likely have consequences for the whole-lake metabolism even beyond the impact to the phototrophic community. IMPORTANCE Autotrophic organisms at the base of the food web are the only life form capable of turning inorganic carbon into the organic form, facilitating the survival of all other organisms. In certain environments, the autotrophic production is limited by environmental conditions and the food web is supported by external carbon inputs. One such environment is stratified boreal lakes, which are one of the biggest natural sources of greenhouse gas emissions in the boreal region. Thus, carbon cycling in these habitats is of utmost importance for the future climate. Here, we demonstrate a high potential for internal carbon cycling via phototrophic and novel chemolithotrophic organisms in the anoxic, poorly illuminated layers of a boreal lake. Our results significantly increase our knowledge on the microbial communities and their metabolic potential in oxygen-depleted freshwaters and help to understand and predict how climate change-induced alterations could impact the lake carbon dynamics.fi
dc.format.mimetypeapplication/pdf
dc.language.isoeng
dc.publisherAmerican Society for Microbiology
dc.relation.ispartofseriesmBio
dc.rightsCC BY 4.0
dc.subject.otherchemoautotrophy
dc.subject.othergreenhouse gas emissions
dc.subject.otheriron oxidizers
dc.subject.othermetagenomics
dc.subject.otherredox gradient
dc.titleNovel Autotrophic Organisms Contribute Significantly to the Internal Carbon Cycling Potential of a Boreal Lake
dc.typearticle
dc.identifier.urnURN:NBN:fi:jyu-201808203857
dc.contributor.laitosBio- ja ympäristötieteiden laitosfi
dc.contributor.laitosDepartment of Biological and Environmental Scienceen
dc.contributor.oppiaineAkvaattiset tieteetfi
dc.contributor.oppiaineAquatic Sciencesen
dc.type.urihttp://purl.org/eprint/type/JournalArticle
dc.date.updated2018-08-20T06:15:10Z
dc.description.reviewstatuspeerReviewed
dc.relation.issn2161-2129
dc.relation.numberinseries4
dc.relation.volume9
dc.type.versionpublishedVersion
dc.rights.copyright© 2018 Peura et al.
dc.rights.accesslevelopenAccessfi
dc.subject.ysohiilen kierto
dc.subject.ysojärvet
dc.subject.ysomikro-organismit
dc.format.contentfulltext
jyx.subject.urihttp://www.yso.fi/onto/yso/p28986
jyx.subject.urihttp://www.yso.fi/onto/yso/p9374
jyx.subject.urihttp://www.yso.fi/onto/yso/p3326
dc.rights.urlhttps://creativecommons.org/licenses/by/4.0/
dc.relation.doi10.1128/mBio.00916-18


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record

CC BY 4.0
Except where otherwise noted, this item's license is described as CC BY 4.0