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dc.contributor.authorAbrahão, Jônatas
dc.contributor.authorSilva, Lorena
dc.contributor.authorOliveira, Danilo
dc.contributor.authorDe Freitas Almeida, Gabriel
dc.date.accessioned2018-06-13T09:42:30Z
dc.date.issued2018fi
dc.identifier.citationAbrahão, J., Silva, L., Oliveira, D., & De Freitas Almeida, G. (2018). Lack of evidence of mimivirus replication in human PBMCs. <em>Microbes and Infection</em>, 20 (5), 281-283. <a href="https://doi.org/10.1016/j.micinf.2018.03.003">doi:10.1016/j.micinf.2018.03.003</a>fi
dc.identifier.otherTUTKAID_77233
dc.identifier.urihttps://jyx.jyu.fi/handle/123456789/58524
dc.description.abstractThe Acanthamoeba polyphaga mimivirus (APMV) was first isolated during a pneumonia outbreak in Bradford, England, and since its discovery many research groups devoted efforts to understand whether this virus could be associated to human diseases, in particular clinical signs and symptoms of pneumonia. In 2013, we observed cytopathic effect in amoebas (rounding and lysis) inoculated with APMV inoculated PBMCs (peripheral blood mononuclear cell) extracts, and at that point we interpreted those results as mimivirus replication in human PBMCs. Based on these results we decided to further investigate APMV replication in human PBMCs, by transmission electron microscopy (TEM) and qPCR. No viral factory was observed in APMV inoculated PBMCs, at any analyzed time and M.O.I.s (multiplicity of infection), by checking 550 cells per condition tested. We also measured the variation of viral DNA by qPCR targeting helicase gene during the course of the TEM experiment in PBMCs, but the DNA levels stayed the same as the first time-point post infection. In summary, our newest qPCR and TEM results do not support previous statements (including ours) that mimivirus is able to replicate in humans PBMCs.fi
dc.format.mimetypeapplication/pdf
dc.language.isoeng
dc.publisherElsevier Masson
dc.relation.ispartofseriesMicrobes and Infection
dc.rightsCC BY-NC-ND 4.0
dc.subject.otherviruksetfi
dc.subject.othermimivirusfi
dc.subject.otherPBMCsfi
dc.subject.otheracanthamoebafi
dc.titleLack of evidence of mimivirus replication in human PBMCsfi
dc.typearticle
dc.identifier.urnURN:NBN:fi:jyu-201806053016
dc.contributor.laitosBio- ja ympäristötieteiden laitosfi
dc.contributor.laitosThe Department of Biological and Environmental Scienceen
dc.contributor.oppiaineSolu- ja molekyylibiologia
dc.date.embargo2019-06-01
dc.type.urihttp://purl.org/eprint/type/JournalItem
dc.identifier.doi10.1016/j.micinf.2018.03.003
dc.date.updated2018-06-05T12:15:09Z
dc.description.reviewstatusnonPeerReviewed
dc.format.pagerange281-283
dc.relation.issn1286-4579
dc.relation.numberinseries5
dc.relation.volume20
dc.type.versionFinal Draft
dc.rights.copyright© 2018 Institut Pasteur. Published by Elsevier Masson SAS.
dc.rights.accesslevelopenAccessfi
dc.format.contentfulltext
dc.rights.urlhttps://creativecommons.org/licenses/by-nc-nd/4.0/


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