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Excitation-Wavelength Dependent Photocycle Initiation Dynamics Resolve Heterogeneity in the Photoactive Yellow Protein from Halorhodospira halophila

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Mix, L. T., Carroll, E. C., Morozov, D., Pan, J., Gordon, W. R., Philip, A., Fuzell, J., Kumauchi, M., van Stokkum, I., Groenhof, G., Hoff, W. D., & Larsen, D. S. (2018). Excitation-Wavelength Dependent Photocycle Initiation Dynamics Resolve Heterogeneity in the Photoactive Yellow Protein from Halorhodospira halophila. Biochemistry, 57(1), 1733-1747. https://doi.org/10.1021/acs.biochem.7b01114
Published in
Biochemistry
Authors
Mix, L. Tyler |
Carroll, Elizabeth C. |
Morozov, Dmitry |
Pan, Jie |
Gordon, Wendy Ryan |
Philip, Andrew |
Fuzell, Jack |
Kumauchi, Masato |
van Stokkum, Ivo |
Groenhof, Gerrit |
Hoff, Wouter D. |
Larsen, Delmar S.
Date
2018
Discipline
Fysikaalinen kemiaNanoscience CenterPhysical ChemistryNanoscience Center
Copyright
© 2018 American Chemical Society. This is a final draft version of an article whose final and definitive form has been published by American Chemical Society. Published in this repository with the kind permission of the publisher

 
Photoactive yellow proteins (PYPs) make up a diverse class of blue-light-absorbing bacterial photoreceptors. Electronic excitation of the p-coumaric acid chromophore covalently bound within PYP results in triphasic quenching kinetics; however, the molecular basis of this behavior remains unresolved. Here we explore this question by examining the excitation-wavelength dependence of the photodynamics of the PYP from Halorhodospira halophila via a combined experimental and computational approach. The fluorescence quantum yield, steady-state fluorescence emission maximum, and cryotrapping spectra are demonstrated to depend on excitation wavelength. We also compare the femtosecond photodynamics in PYP at two excitation wavelengths (435 and 475 nm) with a dual-excitation-wavelength-interleaved pump–probe technique. Multicompartment global analysis of these data demonstrates that the excited-state photochemistry of PYP depends subtly, but convincingly, on excitation wavelength with similar kinetics with distinctly different spectral features, including a shifted ground-state beach and altered stimulated emission oscillator strengths and peak positions. Three models involving multiple excited states, vibrationally enhanced barrier crossing, and inhomogeneity are proposed to interpret the observed excitation-wavelength dependence of the data. Conformational heterogeneity was identified as the most probable model, which was supported with molecular mechanics simulations that identified two levels of inhomogeneity involving the orientation of the R52 residue and different hydrogen bonding networks with the p-coumaric acid chromophore. Quantum calculations were used to confirm that these inhomogeneities track to altered spectral properties consistent with the experimental results. ...
Publisher
American Chemical Society
ISSN Search the Publication Forum
1520-4995
Keywords
Photoactive Yellow Proteins valokemia proteiinit fluoresenssi
DOI
https://doi.org/10.1021/acs.biochem.7b01114
URI

http://urn.fi/URN:NBN:fi:jyu-201804061946

Publication in research information system

https://converis.jyu.fi/converis/portal/detail/Publication/27911135

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  • Matemaattis-luonnontieteellinen tiedekunta [4441]
Related funder(s)
Academy of Finland
Funding program(s)
Research post as Academy Research Fellow, AoF; Others, AoF; Postdoctoral Researcher, AoF; Academy Project, AoF
Additional information about funding
This work was supported by a grant from the National Science Foundation (CHE-1413739) to both D.S.L. and W.D.H. Additionally, W.D.H. acknowledges additional support from National Science Foundation Grants MCB-1051590 and MRI-1338097. G.G. and D.M. acknowledge support from the Academy of Finland (Grants 258806, 290677, and 304455 to G.G. and Grant 285481 to D.M.).

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