Production of High Amounts of Hepatotoxin Nodularin and New Protease Inhibitors Pseudospumigins by the Brazilian Benthic Nostoc sp. CENA543
Jokela, J., Heinilä, L. M. P., Shishido, T. K., Wahlsten, M., Fewer, D. P., Fiore, M. F., Wang, H., Haapaniemi, E., Permi, P., & Sivonen, K. (2017). Production of High Amounts of Hepatotoxin Nodularin and New Protease Inhibitors Pseudospumigins by the Brazilian Benthic Nostoc sp. CENA543. Frontiers in Microbiology, 8, Article 1963. https://doi.org/10.3389/fmicb.2017.01963
Published inFrontiers in Microbiology
DisciplineSolu- ja molekyylibiologiaOrgaaninen kemiaNanoscience CenterCell and Molecular BiologyOrganic ChemistryNanoscience Center
Copyright © 2017 The Authors. This is an open-access article distributed under theterms of the Creative Commons Attribution License.
Nostoc is a cyanobacterial genus, common in soils and a prolific producer of natural products. This research project aimed to explore and characterize Brazilian cyanobacteria for new bioactive compounds. Here we report the production of hepatotoxins and new protease inhibitors from benthic Nostoc sp. CENA543 isolated from a small, shallow, saline-alkaline lake in the Nhecolândia, Pantanal wetland area in Brazil. Nostoc sp. CENA543 produces exceptionally high amounts of nodularin-R. This is the first free-living Nostoc that produces nodularin at comparable levels as the toxic, bloom-forming, Nodularia spumigena. We also characterized pseudospumigins A–F, which are a novel family of linear tetrapeptides. Pseudospumigins are structurally related to linear tetrapeptide spumigins and aeruginosins both present in N. spumigena but differ in respect to their diagnostic amino acid, which is Ile/Leu/Val in pseudospumigins, Pro/mPro in spumigins, and Choi in aeruginosins. The pseudospumigin gene cluster is more similar to the spumigin biosynthetic gene cluster than the aeruginosin gene cluster. Pseudospumigin A inhibited trypsin (IC50 4.5 μM after 1 h) in a similar manner as spumigin E from N. spumigena but was almost two orders of magnitude less potent. This study identifies another location and environment where the hepatotoxic nodularin has the potential to cause the death of eukaryotic organisms. ...
PublisherFrontiers Research Foundation
Publication in research information system
MetadataShow full item record
Except where otherwise noted, this item's license is described as Copyright © 2017 The Authors. This is an open-access article distributed under theterms of the Creative Commons Attribution License.
Showing items with similar title or keywords.
Alternative biosynthetic starter units enhance the structural diversity of cyanobacterial lipopeptides Mareš, Jan; Hájek, Jan; Urajová, Petra; Kust, Andreja; Jokela, Jouni; Saurav, Kumar; Galica, Tomáš; Čapková, Kateřina; Mattila, Antti; Haapaniemi, Esa; Permi, Perttu; Mysterud, Ivar; Skulberg, Olav M.; Karlsen, Jan; Fewer, David P.; Sivonen, Kaarina; Tønnesen, Hanne Hjorth; Hrouzek, Pavel (American Society for Microbiology, 2019)Puwainaphycins (PUWs) and minutissamides (MINs) are structurally analogous cyclic lipopeptides possessing cytotoxic activity. Both types of compound exhibit high structural variability, particularly in the fatty acid (FA) ...
The Biosynthesis of Rare Homo-Amino Acid Containing Variants of Microcystin by a Benthic Cyanobacterium Shishido, Tânia Keiko; Jokela, Jouni; Humisto, Anu; Suurnäkki, Suvi; Wahlsten, Matti; Alvarenga, Danillo O.; Sivonen, Kaarina; Fewer, David P. (MDPI AG, 2019)Microcystins are a family of chemically diverse hepatotoxins produced by distantly related cyanobacteria and are potent inhibitors of eukaryotic protein phosphatases 1 and 2A. Here we provide evidence for the biosynthesis ...
Conservation value of low-productive forests measured as the amount and diversity of dead wood and saproxylic beetles Hämäläinen, Aino; Strengbom, Joachim; Ranius, Thomas (Open Science Centre, University of Jyväskylä, 2018)In many managed landscapes a major part of all remaining unmanaged land is low-productive. Low-productive land is also often over-represented within protected areas, as it is less expensive to set aside. Despite this the ...
Peltomaa, Elina T.; Aalto, Sanni L.; Vuorio, Kristiina M.; Taipale, Sami (Frontiers Media S.A., 2017)The growth and reproduction of animals is affected by their access to resources. In aquatic ecosystems, the availability of essential biomolecules for filter-feeding zooplankton depends greatly on phytoplankton. Here, we ...
Lean internal startups for software product innovation in large companies : Enablers and inhibitors Edison, Henry; Smørsgård, Nina; Wang, Xiaofeng; Abrahamsson, Pekka (Elsevier Inc., 2017)Context: Startups are disrupting traditional markets and replacing well-established actors with their innovative products.To compete in this age of disruption, large and established companies cannot rely on traditional ...