Chromatin organization regulates viral egress dynamics
Aho, V., Myllys, M., Ruokolainen, V., Hakanen, S., Mäntylä, E., Virtanen, J., Hukkanen, V., Kühn, T., Timonen, J., Mattila, K., Larabell, C. A., & Vihinen-Ranta, M. (2017). Chromatin organization regulates viral egress dynamics. Scientific Reports, 7, Article 3692. https://doi.org/10.1038/s41598-017-03630-y
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Scientific ReportsDate
2017Discipline
Solu- ja molekyylibiologiaFysiikkaNanoscience CenterCell and Molecular BiologyPhysicsNanoscience CenterCopyright
© the Authors, 2017. This article is licensed under a Creative Commons Attribution 4.0 International License.
Various types of DNA viruses are known to elicit the formation of a large nuclear viral replication
compartment and marginalization of the cell chromatin. We used three-dimensional soft x-ray
tomography, confocal and electron microscopy, combined with numerical modelling of capsid diffusion
to analyse the molecular organization of chromatin in herpes simplex virus 1 infection and its effect
on the transport of progeny viral capsids to the nuclear envelope. Our data showed that the formation
of the viral replication compartment at late infection resulted in the enrichment of heterochromatin
in the nuclear periphery accompanied by the compaction of chromatin. Random walk modelling of
herpes simplex virus 1–sized particles in a three-dimensional soft x-ray tomography reconstruction of
an infected cell nucleus demonstrated that the peripheral, compacted chromatin restricts viral capsid
diffusion, but due to interchromatin channels capsids are able to reach the nuclear envelope, t
...
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Nature Publishing GroupISSN Search the Publication Forum
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Except where otherwise noted, this item's license is described as © the Authors, 2017. This article is licensed under a Creative Commons Attribution 4.0 International License.