Chromatin organization regulates viral egress dynamics

Abstract
Various types of DNA viruses are known to elicit the formation of a large nuclear viral replication compartment and marginalization of the cell chromatin. We used three-dimensional soft x-ray tomography, confocal and electron microscopy, combined with numerical modelling of capsid diffusion to analyse the molecular organization of chromatin in herpes simplex virus 1 infection and its effect on the transport of progeny viral capsids to the nuclear envelope. Our data showed that the formation of the viral replication compartment at late infection resulted in the enrichment of heterochromatin in the nuclear periphery accompanied by the compaction of chromatin. Random walk modelling of herpes simplex virus 1–sized particles in a three-dimensional soft x-ray tomography reconstruction of an infected cell nucleus demonstrated that the peripheral, compacted chromatin restricts viral capsid diffusion, but due to interchromatin channels capsids are able to reach the nuclear envelope, the site of their nuclear egress.
Main Authors
Format
Articles Research article
Published
2017
Series
Subjects
Publication in research information system
Publisher
Nature Publishing Group
Original source
https://www.nature.com/articles/s41598-017-03630-y.pdf
The permanent address of the publication
https://urn.fi/URN:NBN:fi:jyu-201706192942Use this for linking
Review status
Peer reviewed
ISSN
2045-2322
DOI
https://doi.org/10.1038/s41598-017-03630-y
Language
English
Published in
Scientific Reports
Citation
  • Aho, V., Myllys, M., Ruokolainen, V., Hakanen, S., Mäntylä, E., Virtanen, J., Hukkanen, V., Kühn, T., Timonen, J., Mattila, K., Larabell, C. A., & Vihinen-Ranta, M. (2017). Chromatin organization regulates viral egress dynamics. Scientific Reports, 7, Article 3692. https://doi.org/10.1038/s41598-017-03630-y
License
Open Access
Copyright© the Authors, 2017. This article is licensed under a Creative Commons Attribution 4.0 International License.

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