Chromatin organization regulates viral egress dynamics
Abstract
Various types of DNA viruses are known to elicit the formation of a large nuclear viral replication
compartment and marginalization of the cell chromatin. We used three-dimensional soft x-ray
tomography, confocal and electron microscopy, combined with numerical modelling of capsid diffusion
to analyse the molecular organization of chromatin in herpes simplex virus 1 infection and its effect
on the transport of progeny viral capsids to the nuclear envelope. Our data showed that the formation
of the viral replication compartment at late infection resulted in the enrichment of heterochromatin
in the nuclear periphery accompanied by the compaction of chromatin. Random walk modelling of
herpes simplex virus 1–sized particles in a three-dimensional soft x-ray tomography reconstruction of
an infected cell nucleus demonstrated that the peripheral, compacted chromatin restricts viral capsid
diffusion, but due to interchromatin channels capsids are able to reach the nuclear envelope, the site of
their nuclear egress.
Main Authors
Format
Articles
Research article
Published
2017
Series
Subjects
Publication in research information system
Publisher
Nature Publishing Group
Original source
https://www.nature.com/articles/s41598-017-03630-y.pdf
The permanent address of the publication
https://urn.fi/URN:NBN:fi:jyu-201706192942Use this for linking
Review status
Peer reviewed
ISSN
2045-2322
DOI
https://doi.org/10.1038/s41598-017-03630-y
Language
English
Published in
Scientific Reports
Citation
- Aho, V., Myllys, M., Ruokolainen, V., Hakanen, S., Mäntylä, E., Virtanen, J., Hukkanen, V., Kühn, T., Timonen, J., Mattila, K., Larabell, C. A., & Vihinen-Ranta, M. (2017). Chromatin organization regulates viral egress dynamics. Scientific Reports, 7, Article 3692. https://doi.org/10.1038/s41598-017-03630-y
Copyright© the Authors, 2017. This article is licensed under a Creative Commons Attribution 4.0 International License.