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dc.contributor.authorLeskinen, Tuija
dc.contributor.authorRinnankoski, Rita
dc.contributor.authorRintala, Mirva
dc.contributor.authorSeppänen-Laakso, Tuulikki
dc.contributor.authorLaakkonen, Eija
dc.contributor.authorAlén, Markku
dc.contributor.authorSipilä, Sarianna
dc.contributor.authorKaprio, Jaakko
dc.contributor.authorKovanen, Vuokko
dc.contributor.authorRahkila, Paavo
dc.contributor.authorOresic, Matej
dc.contributor.authorKainulainen, Heikki
dc.contributor.authorKujala, Urho
dc.date.accessioned2017-03-21T11:24:33Z
dc.date.available2017-03-21T11:24:33Z
dc.date.issued2010
dc.identifier.citationLeskinen, T., Rinnankoski, R., Rintala, M., Seppänen-Laakso, T., Laakkonen, E., Alén, M., . . . Kujala, U. (2010). Differences in muscle and adipose tissue gene expression and cardio-metabolic risk factors in the members of physical activity discordant twin pairs. <em>PLoS ONE</em>, 5 (9), e12609. <a href="https://doi.org/10.1371/journal.pone.0012609">doi:10.1371/journal.pone.0012609</a>
dc.identifier.otherTUTKAID_41364
dc.identifier.urihttps://jyx.jyu.fi/handle/123456789/53333
dc.description.abstractHigh physical activity/aerobic fitness predicts low morbidity and mortality. Our aim was to identify the most up-regulated gene sets related to long-term physical activity vs. inactivity in skeletal muscle and adipose tissues and to obtain further information about their link with cardio-metabolic risk factors. We studied ten same-sex twin pairs (age range 50–74 years) who had been discordant for leisure-time physical activity for 30 years. The examinations included biopsies from m. vastus lateralis and abdominal subcutaneous adipose tissue. RNA was analyzed with the genome-wide Illumina Human WG-6 v3.0 Expression BeadChip. For pathway analysis we used Gene Set Enrichment Analysis utilizing active vs. inactive co-twin gene expression ratios. Our findings showed that among the physically active members of twin pairs, as compared to their inactive co-twins, gene expression in the muscle tissue samples was chronically up-regulated for the central pathways related to energy metabolism, including oxidative phosphorylation, lipid metabolism and supportive metabolic pathways. Up-regulation of these pathways was associated in particular with aerobic fitness and high HDL cholesterol levels. In fat tissue we found physical activity-associated increases in the expression of polyunsaturated fatty acid metabolism and branched-chain amino acid degradation gene sets both of which associated with decreased ‘high-risk’ ectopic body fat and plasma glucose levels. Consistent with other findings, plasma lipidomics analysis showed up-regulation of the triacylglycerols containing the polyunsaturated fatty acids. Our findings identified skeletal muscle and fat tissue pathways which are associated with the long-term physical activity and reduced cardio-metabolic disease risk, including increased aerobic fitness. In particular, improved skeletal muscle oxidative energy and lipid metabolism as well as changes in adipocyte function and redistribution of body fat are associated with reduced cardio-metabolic risk.
dc.language.isoeng
dc.publisherPublic Library of Science
dc.relation.ispartofseriesPLoS ONE
dc.subject.otherliikunta
dc.subject.othergeenien ilmentyminen
dc.subject.othermetabolia
dc.subject.otherkaksoset
dc.subject.otherphysical activity
dc.subject.othergene expression
dc.subject.othermatebolism
dc.subject.othertwins
dc.titleDifferences in muscle and adipose tissue gene expression and cardio-metabolic risk factors in the members of physical activity discordant twin pairs.
dc.typearticle
dc.identifier.urnURN:NBN:fi:jyu-201703211715
dc.contributor.laitosLiikuntabiologian laitosfi
dc.contributor.laitosTerveystieteiden laitosfi
dc.contributor.laitosDepartment of Biology of Physical Activityen
dc.contributor.laitosDepartment of Health Sciencesen
dc.contributor.oppiaineLiikuntafysiologia
dc.contributor.oppiaineGerontologia ja kansanterveys
dc.contributor.oppiaineLiikuntalääketiede
dc.type.urihttp://purl.org/eprint/type/JournalArticle
dc.date.updated2017-03-21T10:15:05Z
dc.type.coarjournal article
dc.description.reviewstatuspeerReviewed
dc.relation.issn1932-6203
dc.relation.volume5
dc.type.versionpublishedVersion
dc.rights.copyright© 2010 Leskinen et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License.
dc.rights.accesslevelopenAccessfi
dc.rights.urlhttps://creativecommons.org/licenses/by/4.0/
dc.relation.doi10.1371/journal.pone.0012609


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© 2010 Leskinen et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License.
Except where otherwise noted, this item's license is described as © 2010 Leskinen et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License.