dc.contributor.author | Pelliccia, Maria | |
dc.contributor.author | Andreozzi, Patrizia | |
dc.contributor.author | Paulose, Jayson | |
dc.contributor.author | D’Alicarnasso, Marco | |
dc.contributor.author | Cagno, Valeria | |
dc.contributor.author | Donalisio, Manuela | |
dc.contributor.author | Civra, Andrea | |
dc.contributor.author | Broeckel, Rebecca M. | |
dc.contributor.author | Haese, Nicole | |
dc.contributor.author | Silva, Paulo Jacob | |
dc.contributor.author | Carney, Randy P. | |
dc.contributor.author | Marjomäki, Varpu | |
dc.contributor.author | Streblow, Daniel N. | |
dc.contributor.author | Lembo, David | |
dc.contributor.author | Stellacci, Francesco | |
dc.contributor.author | Vitelli, Vincenzo | |
dc.contributor.author | Krol, Silke | |
dc.date.accessioned | 2016-12-13T08:10:27Z | |
dc.date.available | 2016-12-13T08:10:27Z | |
dc.date.issued | 2016 | |
dc.identifier.citation | Pelliccia, M., Andreozzi, P., Paulose, J., D’Alicarnasso, M., Cagno, V., Donalisio, M., Civra, A., Broeckel, R. M., Haese, N., Silva, P. J., Carney, R. P., Marjomäki, V., Streblow, D. N., Lembo, D., Stellacci, F., Vitelli, V., & Krol, S. (2016). Additives for vaccine storage to improve thermal stability of adenoviruses from hours to months. <i>Nature Communications</i>, <i>7</i>, Article 13520. <a href="https://doi.org/10.1038/ncomms13520" target="_blank">https://doi.org/10.1038/ncomms13520</a> | |
dc.identifier.other | CONVID_26357676 | |
dc.identifier.other | TUTKAID_71947 | |
dc.identifier.uri | https://jyx.jyu.fi/handle/123456789/52286 | |
dc.description.abstract | Up to 80% of the cost of vaccination programmes is due to the cold chain problem (that is,
keeping vaccines cold). Inexpensive, biocompatible additives to slow down the degradation of
virus particles would address the problem. Here we propose and characterize additives that,
already at very low concentrations, improve the storage time of adenovirus type 5. Anionic
gold nanoparticles (10 8–10 6 M) or polyethylene glycol (PEG, molecular weight
B8,000 Da, 10 7–10 4 M) increase the half-life of a green fluorescent protein expressing
adenovirus fromB48 h to 21 days at 37 C (from 7 to430 days at room temperature). They
replicate the known stabilizing effect of sucrose, but at several orders of magnitude lower
concentrations. PEG and sucrose maintained immunogenicity in vivo for viruses stored for 10
days at 37 C. To achieve rational design of viral-vaccine stabilizers, our approach is aided by
simplified quantitative models based on a single rate-limiting step. | |
dc.language.iso | eng | |
dc.publisher | Nature Publishing Group | |
dc.relation.ispartofseries | Nature Communications | |
dc.subject.other | vaccinations | |
dc.subject.other | vaccine storage | |
dc.title | Additives for vaccine storage to improve thermal stability of adenoviruses from hours to months | |
dc.type | article | |
dc.identifier.urn | URN:NBN:fi:jyu-201612054943 | |
dc.contributor.laitos | Bio- ja ympäristötieteiden laitos | fi |
dc.contributor.laitos | Department of Biological and Environmental Science | en |
dc.contributor.oppiaine | Solu- ja molekyylibiologia | fi |
dc.contributor.oppiaine | Nanoscience Center | fi |
dc.contributor.oppiaine | Cell and Molecular Biology | en |
dc.contributor.oppiaine | Nanoscience Center | en |
dc.type.uri | http://purl.org/eprint/type/JournalArticle | |
dc.date.updated | 2016-12-05T10:15:03Z | |
dc.type.coar | http://purl.org/coar/resource_type/c_2df8fbb1 | |
dc.description.reviewstatus | peerReviewed | |
dc.relation.issn | 2041-1723 | |
dc.relation.numberinseries | 0 | |
dc.relation.volume | 7 | |
dc.type.version | publishedVersion | |
dc.rights.copyright | © the Authors 2016. This is an open access article under the terms of the Creative Commons Licence. | |
dc.rights.accesslevel | openAccess | fi |
dc.subject.yso | adenovirukset | |
jyx.subject.uri | http://www.yso.fi/onto/yso/p19797 | |
dc.rights.url | https://creativecommons.org/licenses/by/4.0/ | |
dc.relation.doi | 10.1038/ncomms13520 | |
dc.type.okm | A1 | |