Inducing Cold-Sensitivity in the Frigophilic Fly Drosophila montana by RNAi
Vigoder, F. M., Parker, D., Cook, N., Tournière, O., Sneddon, T., & Richie, M. G. (2016). Inducing Cold-Sensitivity in the Frigophilic Fly Drosophila montana by RNAi. PLoS ONE, 11(11), Article e0165724. https://doi.org/10.1371/journal.pone.0165724
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PLoS ONETekijät
Päivämäärä
2016Tekijänoikeudet
© 2016 Vigoder et al. This is an open access article distributed under the terms of the
Creative Commons Attribution License.
Cold acclimation is a critical physiological adaptation for coping with seasonal cold. By
increasing their cold tolerance individuals can remain active for longer at the onset of winter
and can recover more quickly from a cold shock. In insects, despite many physiological
studies, little is known about the genetic basis of cold acclimation. Recently, transcriptomic
analyses in Drosophila virilis and D. montana revealed candidate genes for cold acclimation
by identifying genes upregulated during exposure to cold. Here, we test the role of myo-inositol-1-phosphate
synthase (Inos), in cold tolerance in D. montana using an RNAi approach.
D. montana has a circumpolar distribution and overwinters as an adult in northern latitudes
with extreme cold. We assessed cold tolerance of dsRNA knock-down flies using two metrics:
chill-coma recovery time (CCRT) and mortality rate after cold acclimation. Injection of
dsRNAInos did not alter CCRT, either overall or in interaction with the cold treatment, however
it did induced cold-specific mortality, with high levels of mortality observed in injected
flies acclimated at 5˚C but not at 19˚C. Overall, injection with dsRNAInos induced a temperature-sensitive
mortality rate of over 60% in this normally cold-tolerant species. qPCR analysis
confirmed that dsRNA injection successfully reduced gene expression of Inos. Thus, our
results demonstrate the involvement of Inos in increasing cold tolerance in D. montana. The
potential mechanisms involved by which Inos increases cold tolerance are also discussed.
...
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