One-step large-scale deposition of salt-free DNA origami nanostructures
Linko, V., Shen, B., Tapio, K., Toppari, J., Kostiainen, M. A., & Tuukkanen, S. (2015). One-step large-scale deposition of salt-free DNA origami nanostructures. Scientific Reports, 5, Article 15634. https://doi.org/10.1038/srep15634
Published inScientific Reports
© 2015 the Authors. Published by Nature Publishing Group. This is an open access article licensed under a Creative Commons Attribution 4.0 International License.
DNA origami nanostructures have tremendous potential to serve as versatile platforms in selfassembly -based nanofabrication and in highly parallel nanoscale patterning. However, uniform deposition and reliable anchoring of DNA nanostructures often requires specific conditions, such as pre-treatment of the chosen substrate or a fine-tuned salt concentration for the deposition buffer. In addition, currently available deposition techniques are suitable merely for small scales. In this article, we exploit a spray-coating technique in order to resolve the aforementioned issues in the deposition of different 2D and 3D DNA origami nanostructures. We show that purified DNA origamis can be controllably deposited on silicon and glass substrates by the proposed method. The results are verified using either atomic force microscopy or fluorescence microscopy depending on the shape of the DNA origami. DNA origamis are successfully deposited onto untreated substrates with surface coverage of about 4 objects/mm2 . Further, the DNA nanostructures maintain their shape even if the salt residues are removed from the DNA origami fabrication buffer after the folding procedure. We believe that the presented one-step spray-coating method will find use in various fields of material sciences, especially in the development of DNA biochips and in the fabrication of metamaterials and plasmonic devices through DNA metallisation. ...
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Except where otherwise noted, this item's license is described as © 2015 the Authors. Published by Nature Publishing Group. This is an open access article licensed under a Creative Commons Attribution 4.0 International License.
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Ramakrishnan, Saminathan; Ijäs, Heini; Linko, Veikko; Keller, Adrian (Research Network of Computational and Structural Biotechnology, 2018)With the introduction of the DNA origami technique, it became possible to rapidly synthesize almost arbitrarily shaped molecular nanostructures at nearly stoichiometric yields. The technique furthermore provides ...
Shen, Boxuan; Tapio, Kosti; Linko, Veikko; Kostiainen, Mauri A.; Toppari, Jussi (MDPI AG, 2016)Metallic nanostructures have inspired extensive research over several decades, particularly within the field of nanoelectronics and increasingly in plasmonics. Due to the limitations of conventional lithography methods, ...
Tapio, Kosti (University of Jyväskylä, 2017)In this thesis DNA based structures were utilized to create gold nanostructures for nanosensing and nanoelectronic applications. In the past, both of these ﬁelds have been dominated by the conventional lithography methods, ...
Parikka, Johannes M.; Sokołowska, Karolina; Markešević, Nemanja; Toppari, J. J. (MDPI, 2021)The predictable nature of deoxyribonucleic acid (DNA) interactions enables assembly of DNA into almost any arbitrary shape with programmable features of nanometer precision. The recent progress of DNA nanotechnology has ...
Linko, Veikko (University of Jyväskylä, 2011)This thesis is mainly focused on DNA molecules and especially on self-assembled DNA constructs and their potential applications in nanotechnology and molecular electronics. In the field of molecular electronics the ...