One-step large-scale deposition of salt-free DNA origami nanostructures
Linko, V., Shen, B., Tapio, K., Toppari, J., Kostiainen, M. A., & Tuukkanen, S. (2015). One-step large-scale deposition of salt-free DNA origami nanostructures. Scientific Reports, 5, Article 15634. https://doi.org/10.1038/srep15634
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Scientific ReportsTekijät
Päivämäärä
2015Tekijänoikeudet
© 2015 the Authors. Published by Nature Publishing Group. This is an open access article licensed under a Creative Commons Attribution 4.0 International License.
DNA origami nanostructures have tremendous potential to serve as versatile platforms in selfassembly
-based nanofabrication and in highly parallel nanoscale patterning. However, uniform
deposition and reliable anchoring of DNA nanostructures often requires specific conditions, such as
pre-treatment of the chosen substrate or a fine-tuned salt concentration for the deposition buffer.
In addition, currently available deposition techniques are suitable merely for small scales. In this
article, we exploit a spray-coating technique in order to resolve the aforementioned issues in the
deposition of different 2D and 3D DNA origami nanostructures. We show that purified DNA origamis
can be controllably deposited on silicon and glass substrates by the proposed method. The results are
verified using either atomic force microscopy or fluorescence microscopy depending on the shape of
the DNA origami. DNA origamis are successfully deposited onto untreated substrates with surface
coverage of about 4 objects/mm2
. Further, the DNA nanostructures maintain their shape even if the
salt residues are removed from the DNA origami fabrication buffer after the folding procedure. We
believe that the presented one-step spray-coating method will find use in various fields of material
sciences, especially in the development of DNA biochips and in the fabrication of metamaterials and
plasmonic devices through DNA metallisation.
...
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Nature Publishing GroupISSN Hae Julkaisufoorumista
2045-2322Asiasanat
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https://converis.jyu.fi/converis/portal/detail/Publication/25271318
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