Functional gene pyrosequencing reveals core proteobacterial denitrifiers in boreal lakes
Saarenheimo, J., Tiirola, M., & Rissanen, A. (2015). Functional gene pyrosequencing reveals core proteobacterial denitrifiers in boreal lakes. Frontiers in Microbiology, 6, Article 674. https://doi.org/10.3389/fmicb.2015.00674
Julkaistu sarjassa
Frontiers in MicrobiologyPäivämäärä
2015Tekijänoikeudet
© 2015 Saarenheimo, Tiirola and Rissanen. This is an open-access article distributed under the terms of the Creative Commons Attribution License.
Denitrification is an important microbial process in aquatic ecosystems that can reduce
the effects of eutrophication. Here, quantification and pyrosequencing of nirS, nirK,
and nosZ genes encoding for nitrite and nitrous oxide reductases was performed in
sediment samples from four boreal lakes to determine the structure and seasonal
stability of denitrifying microbial populations. Sediment quality and nitrate concentrations
were linked to the quantity and diversity of denitrification genes, the abundance of
denitrifying populations (nirS and nosZ genes) correlated with coupled nitrificationdenitrification
(Dn), and the denitrification of the overlying water NO3
− (Dw) correlated
with the nirS/nirK ratio. The number of core nirS, nirK, and nosZ operational taxonomical
units (OTUs) was low (6, 7, and 3, respectively), and most of these core OTUs
were shared among the lakes. Dominant nirK sequences matched best with those
of the order Rhizobiales, which was one of the main bacterial orders present in the
sediment microbiomes, whereas the dominant nirS sequences were affiliated with the
order Burkholderiales. Over half of the nosZ sequences belonged to a single OTU of
the order Burkholderiales, but coupled nitrification–denitrification rate correlated with
another dominant nosZ OTU assigned to the order Rhodospirillales. The study indicates
that a few core proteobacterial clusters may drive denitrification in boreal lake sediments,
as the same Alpha- and Betaproteobacteria denitrifier clusters were present in different
lakes and seasons.
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Frontiers Research FoundationISSN Hae Julkaisufoorumista
1664-302XAsiasanat
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https://converis.jyu.fi/converis/portal/detail/Publication/24797593
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