Hormone replacement therapy enhances IGF-1 signaling in skeletal muscle by diminishing miR-182 and miR-233 expressions: A study on postmenopausal monozygotic twin pairs
Olivieri, F., Ahtiainen, M., Lazzarini, R., Laakkonen, E., Capri, M., Lorenzi, M., Fulgenzi, G., Albertini, M. C., Salvioli, S., Alen, M. J., Kujala, U., Borghetti, G., Babini, L., Kaprio, J., Sipilä, S., Franceschi, C., Kovanen, V., & Procopio, A. D. (2014). Hormone replacement therapy enhances IGF-1 signaling in skeletal muscle by diminishing miR-182 and miR-233 expressions: A study on postmenopausal monozygotic twin pairs. Aging Cell, 13(5), 850-861. https://doi.org/10.1111/acel.12245
Julkaistu sarjassa
Aging CellTekijät
Päivämäärä
2014Oppiaine
Gerontologia ja kansanterveysLiikuntalääketiedeGerontologian tutkimuskeskusHyvinvoinnin tutkimuksen yhteisöGerontology and Public HealthSports and Exercise MedicineGerontology Research CenterSchool of WellbeingTekijänoikeudet
© The Authors. Aging Cell published by the Anatomical Society and John Wiley & Sons Ltd.
This is an open access article under the terms of the Creative Commons Attribution License, which permits use,
distribution and reproduction in any medium, provided the original work is properly cited.
MiRNAs are fine-tuning modifiers of skeletal muscle regulation,
but knowledge of their hormonal control is lacking. We used a
co-twin case–control study design, that is, monozygotic postmenopausal
twin pairs discordant for estrogen-based hormone
replacement therapy (HRT) to explore estrogen-dependent
skeletal muscle regulation via miRNAs. MiRNA profiles were
determined from vastus lateralis muscle of nine healthy 54–62-
years-old monozygotic female twin pairs discordant for HRT
(median 7 years). MCF-7 cells, human myoblast cultures and
mouse muscle experiments were used to confirm estrogen’s
causal role on the expression of specific miRNAs, their target
mRNAs and proteins and finally the activation of related
signaling pathway. Of the 230 miRNAs expressed at detectable
levels in muscle samples, qPCR confirmed significantly lower
miR-182, miR-223 and miR-142-3p expressions in HRT using than
in their nonusing co-twins. Insulin/IGF-1 signaling emerged one
common pathway targeted by these miRNAs. IGF-1R and FOXO3A
mRNA and protein were more abundantly expressed in muscle
samples of HRT users than nonusers. In vitro assays confirmed
effective targeting of miR-182 and miR-223 on IGF-1R and FOXO3A
mRNA as well as a dose-dependent miR-182 and miR-223 downregulations
concomitantly with up-regulation of FOXO3A and IGF-
1R expression. Novel finding is the postmenopausal HRT-reduced
miRs-182, miR-223 and miR-142-3p expression in female skeletal
muscle. The observed miRNA-mediated enhancement of the target
genes’ IGF-1R and FOXO3A expression as well as the activation of
insulin/IGF-1 pathway signaling via phosphorylation of AKT and
mTOR is an important mechanism for positive estrogen impact on
skeletal muscle of postmenopausal women.
...
Julkaisija
Wiley-Blackwell; Anatomical SocietyISSN Hae Julkaisufoorumista
1474-9718Asiasanat
Alkuperäislähde
http://onlinelibrary.wiley.com/doi/10.1111/acel.12245/pdfJulkaisu tutkimustietojärjestelmässä
https://converis.jyu.fi/converis/portal/detail/Publication/23750600
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This is an open access article under the terms of the Creative Commons Attribution License, which permits use,
distribution and reproduction in any medium, provided the original work is properly cited.
Samankaltainen aineisto
Näytetään aineistoja, joilla on samankaltainen nimeke tai asiasanat.
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Global gene expression profiles in skeletal muscle of monozygotic female twins discordant for hormone replacement therapy.
Ronkainen, Paula; Laakkonen, Eija; Alén, Markku; Pitkänen, Reino; Puolakka, Jukka; Kujala, Urho; Kaprio, Jaakko; Sipilä, Sarianna; Kovanen, Vuokko (Blackwell Publishing Ltd/Anatomical Society of Great Britain and Ireland, 2010)Aging is accompanied by inexorable loss of muscle tissue. One of the underlying causes for this is the massive change in the hormonal milieu of the body. The role of a female sex steroid – estrogen – in these processes is ... -
Regulation of gene expression and steroidogenesis in skeletal muscle of postmenopausal women : with emphasis on the effects of hormone replacement and power training
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Postmenopausal Hormone Replacement Therapy Modifies Skeletal Muscle Composition and Function: A Study with Monozygotic Twin Pairs
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Circulating miR-21, miR-146a and Fas ligand respond to postmenopausal estrogen-based hormone replacement therapy : A study with monozygotic twin pairs
Kangas, Reeta; Pöllänen, Eija; Rippo, Maria Rita; Lanzarini, Catia; Prattichizzo, Francesco; Niskala, Paula; Jylhävä, Juulia; Sipilä, Sarianna; Kaprio, Jaakko; Procopio, Antonio Domenico; Capri, Miriam; Franceschi, Claudio; Olivieri, Fabiola; Kovanen, Vuokko (Elsevier Ireland Ltd., 2014)Biological aging is associated with physiological deteriorations and its’ remodeling, which are partly due to changes in the hormonal profile. MicroRNAs are known to post-transcriptionally regulate various cellular processes ... -
Influence of long-term postmenopausal hormone replacement therapy on estimated structural bone strength: A study in discordant monozygotic twins.
Mikkola, Tuija; Heinonen, Ari; Kovanen, Vuokko; Cheng, Sulin; Kujala, Urho; Suominen, Harri; Alén, Markku; Puolakka, Jukka; Ankarberg-Lindgren, Carina; Ronkainen, Paula; Koskenvuo, Markku; Kaprio, Jaakko; Rantanen, Taina; Sipilä, Sarianna (Wiley Blackwell, 2011)Although postmenopausal hormone-replacement therapy (HRT) is known to prevent fractures, knowledge on the influence of long-term HRT on bone strength and its determinants other than areal bone mineral density is scarce. ...
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