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dc.contributor.authorTakala, Heikki
dc.contributor.authorYlänne, Jari
dc.date.accessioned2012-08-21T11:46:54Z
dc.date.available2012-08-21T11:46:54Z
dc.date.issued2012
dc.identifier.citationTakala H, Ylänne J (2012) Binding Properties and Stability of the Ras-Association Domain of Rap1-GTP Interacting Adapter Molecule (RIAM). PLoS ONE 7(4): e31955. doi:10.1371/journal.pone.0031955
dc.identifier.urihttps://jyx.jyu.fi/handle/123456789/38344
dc.description.abstractThe Rap1-GTP interacting adapter protein (RIAM) is an important protein in Rap1-mediated integrin activation. By binding to both Rap1 GTPase and talin, RIAM recruits talin to the cell membrane, thus facilitating talin-dependent integrin activation. In this article, we studied the role of the RIAM Ras-association (RA) and pleckstrin-homology (PH) domains in the interaction with Rap1. We found that the RA domain was sufficient for GTP-dependent interaction with Rap1B, and the addition of the PH domain did not change the binding affinity. We also detected GTP-independent interaction of Rap1B with the N-terminus of RIAM. In addition, we found that the PH domain stabilized the RA domain both in vitro and in cells.fi
dc.language.isoeng
dc.publisherPublic Library of Science
dc.relation.ispartofseriesPLoS ONE
dc.subject.otherinteractionen
dc.subject.otherintegrinsen
dc.subject.otherRIAMfi
dc.subject.othersitoutuminenfi
dc.subject.otherintegriinitfi
dc.titleBinding Properties and Stability of the Ras-Association Domain of Rap1-GTP Interacting Adapter Molecule (RIAM)
dc.typeArticle
dc.identifier.urnURN:NBN:fi:jyu-201208212213
dc.contributor.laitosBio- ja ympäristötieteiden laitosfi
dc.contributor.laitosThe Department of Biological and Environmental Scienceen
dc.type.urihttp://purl.org/eprint/type/JournalArticle
dc.type.coarjournal article
dc.description.reviewstatuspeerReviewed
dc.relation.issn1932-6203
dc.type.versionpublishedVersion
dc.rights.copyright© 2012 Takala, Ylänne. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
dc.rights.accesslevelopenAccessfi
dc.rights.urlhttps://creativecommons.org/licenses/by/2.0/
dc.relation.doidoi:10.1371/journal.pone.0031955


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© 2012 Takala, Ylänne. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Except where otherwise noted, this item's license is described as © 2012 Takala, Ylänne. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.