Näytä suppeat kuvailutiedot

dc.contributor.authorKinnunen, Paula M.
dc.contributor.authorInkeroinen, Hanna
dc.contributor.authorIlander, Mette
dc.contributor.authorHeikkilä, Henna P.
dc.contributor.authorKallio, Eva
dc.contributor.authorKoskela, Esa
dc.contributor.authorMappes, Tapio
dc.contributor.authorPalva, Airi
dc.contributor.authorVaheri, Antti
dc.contributor.authorKipar, Anja
dc.contributor.authorVapalahti, Olli
dc.date.accessioned2012-08-21T10:24:24Z
dc.date.available2012-08-21T10:24:24Z
dc.date.issued2011
dc.identifier.citationKinnunen PM, Inkeroinen H, Ilander M, Kallio ER, Heikkilä HP, et al. (2011) Intracerebral Borna Disease Virus Infection of Bank Voles Leading to Peripheral Spread and Reverse Transcription of Viral RNA. PLoS ONE 6(8): e23622. doi:10.1371/journal.pone.0023622
dc.identifier.urihttps://jyx.jyu.fi/handle/123456789/38342
dc.description.abstractBornaviruses, which chronically infect many species, can cause severe neurological diseases in some animal species; their association with human neuropsychiatric disorders is, however, debatable. The epidemiology of Borna disease virus (BDV), as for other members of the family Bornaviridae, is largely unknown, although evidence exists for a reservoir in small mammals, for example bank voles (Myodes glareolus). In addition to the current exogenous infections and despite the fact that bornaviruses have an RNA genome, bornavirus sequences integrated into the genomes of several vertebrates millions of years ago. Our hypothesis is that the bank vole, a common wild rodent species in traditional BDV-endemic areas, can serve as a viral host; we therefore explored whether this species can be infected with BDV, and if so, how the virus spreads and whether viral RNA is transcribed into DNA in vivo. We infected neonate bank voles intracerebrally with BDV and euthanized them 2 to 8 weeks post-infection. Specific Ig antibodies were detectable in 41%. Histological evaluation revealed no significant pathological alterations, but BDV RNA and antigen were detectable in all infected brains. Immunohistology demonstrated centrifugal spread throughout the nervous tissue, because viral antigen was widespread in peripheral nerves and ganglia, including the mediastinum, esophagus, and urinary bladder. This was associated with viral shedding in feces, of which 54% were BDV RNA-positive, and urine at 17%. BDV nucleocapsid gene DNA occurred in 66% of the infected voles, and, surprisingly, occasionally also phosphoprotein DNA. Thus, intracerebral BDV infection of bank vole led to systemic infection of the nervous tissue and viral excretion, as well as frequent reverse transcription of the BDV genome, enabling genomic integration. This first experimental bornavirus infection in wild mammals confirms the recent findings regarding bornavirus DNA, and suggests that bank voles are capable of bornavirus transmission.fi
dc.language.isoeng
dc.publisherPublic Library of Science
dc.relation.ispartofseriesPLoS ONE
dc.subject.otherbornavirusfi
dc.subject.otherviruksetfi
dc.titleIntracerebral Borna disease virus infection of bank voles leading to peripheral spread and reverse transcription of viral RNA
dc.typeArticle
dc.identifier.urnURN:NBN:fi:jyu-201208212211
dc.contributor.laitosBio- ja ympäristötieteiden laitosfi
dc.contributor.laitosThe Department of Biological and Environmental Scienceen
dc.type.urihttp://purl.org/eprint/type/JournalArticle
dc.identifier.doidoi:10.1371/journal.pone.0023622
dc.type.coarjournal article
dc.description.reviewstatuspeerReviewed
dc.relation.issn1932-6203
dc.type.versionpublishedVersion
dc.rights.copyright© 2011 Kinnunen et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
dc.rights.accesslevelopenAccessfi
dc.rights.urlhttps://creativecommons.org/licenses/by/2.0/


Aineistoon kuuluvat tiedostot

TiedostotKokoTiedostomuotoKatso

Aineistoon ei liity tiedostoja.

Aineisto kuuluu seuraaviin kokoelmiin

Näytä suppeat kuvailutiedot

© 2011 Kinnunen et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Ellei muuten mainita, aineiston lisenssi on © 2011 Kinnunen et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.