Exercise-induced expression of angiogenic growth factors in skeletal muscle and in capillaries of healthy and diabetic mice

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dc.contributor.author Kivelä, Riikka
dc.contributor.author Silvennoinen, Mika
dc.contributor.author Lehti, Maarit
dc.contributor.author Jalava, Sanni
dc.contributor.author Vihko, Veikko
dc.contributor.author Kainulainen, Heikki
dc.date.accessioned 2012-11-20T11:57:25Z
dc.date.available 2012-11-20T11:57:25Z
dc.date.issued 2008-05-01 fi
dc.identifier.citation Kivelä, R., Silvennoinen, M., Lehti, M., Jalava, S., Vihko, V., & Kainulainen, H. (2008). Exercise-induced expression of angiogenic growth factors in skeletal muscle and in capillaries of healthy and diabetic mice. Cardiovascular Diabetology, 7 (13). Retrieved from http://www.cardiab.com/content/7/1/13 fi
dc.identifier.issn 1471-2148
dc.identifier.uri http://dx.doi.org/10.1186/1475-2840-7-13
dc.identifier.uri http://hdl.handle.net/123456789/40383
dc.description.abstract Background. Diabetes has negative, and exercise training positive, effects on the skeletal muscle vasculature, but the mechanisms are not yet fully understood. In the present experiment the effects of running exercise on the mRNA expression of pro- and antiangiogenic factors were studied in healthy and diabetic skeletal muscle. The responses in capillaries and muscle fibers, collected from the muscle with laser capture microdissection, were also studied separately. Methods. Healthy and streptozotocin-induced diabetic mice were divided into sedentary and exercise groups. Exercise was a single bout of 1 h running on a treadmill. Gastrocnemius muscles were harvested 3 h and 6 h post exercise, and angiogenesis-related gene expressions were analyzed with real-time PCR. In addition to muscle homogenates, capillaries and muscle fibers were collected from the muscle with laser capture microdissection method and analyzed for vascular endothelial growth factor-A (VEGF-A) and thrombospondin-1 (TSP-1) mRNA expression. Results. Of the proangiogenic factors, VEGF-A and VEGF receptor-2 (VEGFR-2) mRNA expression increased significantly (P < 0.05) in healthy skeletal muscle 6 h post exercise. VEGF-B also showed a similar trend (P = 0.08). No significant change was observed post exercise in diabetic muscles in the expression of VEGF-A, VEGFR-2 or VEGF-B. The expression of angiogenesis inhibitor TSP-1 and angiogenic extracellular matrix protein Cyr61 were significantly increased in diabetic muscles (P < 0.05–0.01). Capillary mRNA expression resembled that in the muscle homogenates, however, the responses were greater in capillaries compared to muscle homogenates and pure muscle fibers. Conclusion. The present study is the first to report the effects of a single bout of exercise on the expression of pro- and antiangiogenic factors in diabetic skeletal muscle, and it provides novel data about the separate responses in capillaries and muscle fibers to exercise and diabetes. Diabetic mice seem to have lower angiogenic responses to exercise compared to healthy mice, and they show markedly increased expression of angiogenesis inhibitor TSP-1. Furthermore, exercise-induced VEGF-A expression was shown to be greater in capillaries than in muscle fibers. fi
dc.language.iso eng
dc.publisher BioMed Central (BMC)
dc.relation.ispartofseries Cardiovascular Diabetology
dc.rights © 2008 Kivelä et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
dc.rights.uri http://creativecommons.org/licenses/by/2.0
dc.subject.other diabetes
dc.subject.other kapillaari
dc.subject.other liikunta
dc.subject.other geeniekspressio
dc.title Exercise-induced expression of angiogenic growth factors in skeletal muscle and in capillaries of healthy and diabetic mice fi
dc.type Article en
dc.subject.kota 315
dc.contributor.laitos Liikuntabiologian laitos fi
dc.contributor.laitos Department of Biology of Physical Activity en
dc.type.uri http://purl.org/eprint/type/JournalArticle
dc.identifier.doi 10.1186/1475-2840-7-13
dc.date.updated 2012-11-15T14:54:25Z
dc.description.version Published version
eprint.status http://purl.org/eprint/type/status/PeerReviewed
dc.rights.holder Riikka Kivelä et al.; licensee BioMed Central Ltd.

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