Effects of high-fat diet and physical activity on pyruvate dehydrogenase kinase-4 in mouse skeletal muscle

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dc.contributor.author Rinnankoski-Tuikka, Rita
dc.contributor.author Silvennoinen, Mika
dc.contributor.author Torvinen, Sira
dc.contributor.author Hulmi, Juha J.
dc.contributor.author Lehti, Maarit
dc.contributor.author Kivelä, Riikka
dc.contributor.author Reunanen, Hilkka
dc.contributor.author Kainulainen, Heikki
dc.date.accessioned 2012-11-16T08:40:34Z
dc.date.available 2012-11-16T08:40:34Z
dc.date.issued 2012-06-09
dc.identifier.citation Rinnankoski-Tuikka, R., Silvennoinen, M., Torvinen, S., Hulmi, J., Lehti, M., Kivelä, R., . . . , & Kainulainen, H. (2012). Effects of high-fat diet and physical activity on pyruvate dehydrogenase kinase-4 in mouse skeletal muscle. Nutrition & Metabolism, 9 (53). doi:10.1186/1743-7075-9-53 Retrieved from http://www.nutritionandmetabolism.com/content/9/1/53/abstract
dc.identifier.issn 1743-7075
dc.identifier.uri http://dx.doi.org/10.1186/1743-7075-9-53
dc.identifier.uri http://hdl.handle.net/123456789/40343
dc.description.abstract Background. The expression of PDK4 is elevated by diabetes, fasting and other conditions associated with the switch from the utilization of glucose to fatty acids as an energy source. It is previously shown that peroxisome proliferator-activated receptor γ coactivator 1α (PGC-1α), a master regulator of energy metabolism, coactivates in cell lines pyruvate dehydrogenase kinase-4 (PDK4) gene expression via the estrogen-related receptor α (ERRα). We investigated the effects of long-term high-fat diet and physical activity on the expression of PDK4, PGC-1α and ERRα and the amount and function of mitochondria in skeletal muscle. Methods. Insulin resistance was induced by a high-fat (HF) diet for 19 weeks in C57BL/6 J mice, which were either sedentary or with access to running wheels. The skeletal muscle expression levels of PDK4, PGC-1α and ERRα were measured and the quality and quantity of mitochondrial function was assessed. Results. The HF mice were more insulin-resistant than the low-fat (LF) -fed mice. Upregulation of PDK4 and ERRα mRNA and protein levels were seen after the HF diet, and when combined with running even more profound effects on the mRNA expression levels were observed. Chronic HF feeding and voluntary running did not have significant effects on PGC-1α mRNA or protein levels. No remarkable difference was found in the amount or function of mitochondria. Conclusions. Our results support the view that insulin resistance is not mediated by the decreased qualitative or quantitative properties of mitochondria. Instead, the role of PDK4 should be contemplated as a possible contributor to high-fat diet-induced insulin resistance.
dc.language.iso eng
dc.publisher BioMed Central (BMC)
dc.relation.ispartofseries Nutrition & Metabolism
dc.rights © 2012 Rinnankoski-Tuikka et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
dc.rights.uri http://creativecommons.org/licenses/by/2.0
dc.subject.other lihas fi
dc.subject.other mitokondria fi
dc.subject.other lipidit fi
dc.subject.other glukoosi fi
dc.subject.other skeletal muscle en
dc.subject.other mitochondria en
dc.subject.other lipids en
dc.subject.other glucose en
dc.subject.other fuel switching en
dc.title Effects of high-fat diet and physical activity on pyruvate dehydrogenase kinase-4 in mouse skeletal muscle
dc.type Article en
dc.subject.kota 315
dc.contributor.laitos Bio- ja ympäristötieteiden laitos fi
dc.contributor.laitos The Department of Biological and Environmental Science en
dc.contributor.laitos Liikuntabiologian laitos fi
dc.contributor.laitos Department of Biology of Physical Activity en
dc.type.uri http://purl.org/eprint/type/JournalArticle
dc.identifier.doi 10.1186/1743-7075-9-53
dc.date.updated 2012-11-13T08:29:04Z
dc.description.version Published version
eprint.status http://purl.org/eprint/type/status/PeerReviewed
dc.rights.holder Rita Rinnankoski-Tuikka et al.; licensee BioMed Central Ltd.

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