Changes in gene expression linked with adult reproductive diapause in a northern malt fly species: a candidate gene microarray study

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dc.contributor.author Kankare, Maaria
dc.contributor.author Salminen, Tiina
dc.contributor.author Laiho, Asta
dc.contributor.author Vesala, Laura
dc.contributor.author Hoikkala, Anneli
dc.date.accessioned 2012-08-20T08:13:50Z
dc.date.available 2012-08-20T08:13:50Z
dc.date.issued 2010
dc.identifier.citation Kankare, M., Salminen, T., Laiho, A., Vesala, L., & Hoikkala, A. (2010). Changes in gene expression linked with adult reproductive diapause in a northern malt fly species: a candidate gene microarray study. BMC Ecology, 2010 (10), 3-9. Retrieved from http://www.biomedcentral.com/1472-6785/10/3
dc.identifier.issn 1472-6785
dc.identifier.uri http://hdl.handle.net/123456789/38310
dc.description.abstract Background: Insect diapause is an important biological process which involves many life-history parameters important for survival and reproductive fitness at both individual and population level. Drosophila montana, a species of D. virilis group, has a profound photoperiodic reproductive diapause that enables the adult flies to survive through the harsh winter conditions of high latitudes and altitudes. We created a custom-made microarray for D. montana with 101 genes known to affect traits important in diapause, photoperiodism, reproductive behaviour, circadian clock and stress tolerance in model Drosophila species. This array gave us a chance to filter out genes showing expression changes during photoperiodic reproductive diapause in a species adapted to live in northern latitudes with high seasonal changes in environmental conditions. Results: Comparisons among diapausing, reproducing and young D. montana females revealed expression changes in 24 genes on microarray; for example in comparison between diapausing and reproducing females one gene (Drosophila cold acclimation gene, Dca) showed up-regulation and 15 genes showed down-regulation in diapausing females. Down-regulation of seven of these genes was specific to diapause state while in five genes the expression changes were linked with the age of the females rather than with their reproductive status. Also, qRT-PCR experiments confirmed couch potato (cpo) gene to be involved in diapause of D. montana. Conclusions: A candidate gene microarray proved to offer a practical and cost-effective way to trace genes that are likely to play an important role in photoperiodic reproductive diapause and further in adaptation to seasonally varying environmental conditions. The present study revealed two genes, Dca and cpo, whose role in photoperiodic diapause in D. montana is worth of studying in more details. Also, further studies using the candidate gene microarray with more specific experimental designs and target tissues may reveal additional genes with more restricted expression patterns. fi
dc.language.iso eng
dc.publisher BioMed Central (BMC)
dc.relation.ispartofseries BMC Ecology
dc.relation.uri http://www.biomedcentral.com/1472-6785/10/3
dc.rights © 2010 Kankare et al; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
dc.subject.other geenit fi
dc.subject.other lisääntymislepotila fi
dc.subject.other mikrosiru fi
dc.subject.other gene expression en
dc.subject.other reproductive diapause en
dc.subject.other microarray en
dc.title Changes in gene expression linked with adult reproductive diapause in a northern malt fly species: a candidate gene microarray study
dc.type Article en
dc.identifier.urn URN:NBN:fi:jyu-201208202180
dc.subject.kota 118
dc.contributor.laitos Bio- ja ympäristötieteiden laitos fi
dc.contributor.laitos The Department of Biological and Environmental Science en
dc.type.uri http://purl.org/eprint/type/JournalArticle
dc.identifier.doi doi:10.1186/1472-6785-10-3
dc.description.version Publisher's PDF
eprint.status http://purl.org/eprint/type/status/PeerReviewed

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