Näytä suppeat kuvailutiedot

dc.contributor.authorMarttila, Ari
dc.date.accessioned2008-01-09T12:52:58Z
dc.date.available2008-01-09T12:52:58Z
dc.date.issued2002
dc.identifier.isbn951-39-1283-3
dc.identifier.otheroai:jykdok.linneanet.fi:880749
dc.identifier.urihttps://jyx.jyu.fi/handle/123456789/13172
dc.description.abstractAvidin is a tetrameric glycoprotein found in chicken egg white. Due to its remarkably high affinity for biotin, avidin has widely been used in biological and biotechnical applications in life sciences. Avidin is also a highly stable protein, maintaining its functional tetrameric structure at high temperatures, extremes of pH, and in the presence of strong denaturants. In the present study we wanted to find out whether different properties of avidin can be modified with protein engineering using rational design. We used the 3D-structure of avidin together with sequence information from various avidin-like proteins to plan these changes. Besides shedding more light on the protein chemical bases for the high-affinity binding, stability or the physicochemical characteristics of avidin, our aim was also to create new tools for the avidin-biotin technology.en
dc.format.extentverkkoaineisto.
dc.language.isoeng
dc.publisherUniversity of Jyväskylä
dc.relation.ispartofseriesJyväskylä studies in biological and environmental science
dc.relation.isversionofMyös painettuna.
dc.subject.otherbiotiinisitominen
dc.subject.otheroligomerisaatio
dc.subject.otheroligomerisaatio
dc.titleEngineering of charge, biotin-binding and oligomerization of avidin : new tools for avidin-biotin technology
dc.typeDiss.
dc.identifier.urnURN:ISBN:951-39-1283-3
dc.type.dcmitypeTexten
dc.type.ontasotVäitöskirjafi
dc.type.ontasotDoctoral dissertationen
dc.contributor.tiedekuntaMatemaattis-luonnontieteellinen tiedekuntafi
dc.contributor.tiedekuntaFaculty of Mathematics and Scienceen
dc.contributor.yliopistoUniversity of Jyväskyläen
dc.contributor.yliopistoJyväskylän yliopistofi
dc.contributor.oppiaineMolekyylibiologiafi
dc.relation.issn1456-9701
dc.relation.numberinseries112
dc.rights.accesslevelopenAccessfi
dc.subject.ysoproteiinit
dc.subject.ysoaminohapot
dc.subject.ysomolekyylibiologia
dc.subject.ysoorgaaniset yhdisteet
dc.subject.ysomuokkaus
dc.subject.ysomuutokset
dc.subject.ysoavidiini


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