Engineering of charge, biotin-binding and oligomerization of avidin : new tools for avidin-biotin technology

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dc.contributor.author Marttila, Ari
dc.date.accessioned 2008-01-09T12:52:58Z
dc.date.available 2008-01-09T12:52:58Z
dc.date.issued 2002
dc.identifier.isbn 951-39-1283-3
dc.identifier.uri http://urn.fi/URN:ISBN:951-39-1283-3
dc.identifier.uri http://hdl.handle.net/123456789/13172
dc.description.abstract Avidin is a tetrameric glycoprotein found in chicken egg white. Due to its remarkably high affinity for biotin, avidin has widely been used in biological and biotechnical applications in life sciences. Avidin is also a highly stable protein, maintaining its functional tetrameric structure at high temperatures, extremes of pH, and in the presence of strong denaturants. In the present study we wanted to find out whether different properties of avidin can be modified with protein engineering using rational design. We used the 3D-structure of avidin together with sequence information from various avidin-like proteins to plan these changes. Besides shedding more light on the protein chemical bases for the high-affinity binding, stability or the physicochemical characteristics of avidin, our aim was also to create new tools for the avidin-biotin technology. en
dc.language.iso eng
dc.publisher University of Jyväskylä
dc.relation.ispartofseries Jyväskylä studies in biological and environmental science;1456-9701 ;112
dc.title Engineering of charge, biotin-binding and oligomerization of avidin : new tools for avidin-biotin technology
dc.type Diss. fi
dc.identifier.urn URN:ISBN:951-39-1283-3
dc.subject.ysa proteiinit
dc.subject.ysa aminohapot
dc.subject.ysa molekyylibiologia
dc.subject.ysa orgaaniset yhdisteet
dc.type.dcmitype Text en
dc.type.ontasot Väitöskirja fi
dc.type.ontasot Doctoral dissertation en
dc.contributor.tiedekunta Matemaattis-luonnontieteellinen tiedekunta fi
dc.contributor.tiedekunta Faculty of Mathematics and Science en
dc.contributor.yliopisto University of Jyväskylä en
dc.contributor.yliopisto Jyväskylän yliopisto fi

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